RGD Reference Report - Growth-inhibitory activity and downregulation of the class II tumor-suppressor gene H-rev107 in tumor cell lines and experimental tumors. - Rat Genome Database

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Growth-inhibitory activity and downregulation of the class II tumor-suppressor gene H-rev107 in tumor cell lines and experimental tumors.

Authors: Sers, C  Emmenegger, U  Husmann, K  Bucher, K  Andres, AC  Schafer, R 
Citation: Sers C, etal., J Cell Biol. 1997 Feb 24;136(4):935-44.
RGD ID: 2290001
Pubmed: PMID:9049257   (View Abstract at PubMed)
PMCID: PMC2132501   (View Article at PubMed Central)

The H-rev107 gene is a new class II tumor suppressor, as defined by its reversible downregulation and growth-inhibiting capacity in HRAS transformed cell lines. Overexpression of the H-rev107 cDNA in HRAS-transformed ANR4 hepatoma cells or in FE-8 fibroblasts resulted in 75% reduction of colony formation. Cell populations of H-rev107 transfectants showed an attenuated tumor formation in nude mice. Cells explanted from tumors or maintained in cell culture for an extended period of time no longer exhibited detectable levels of the H-rev107 protein, suggesting strong selection against H-rev107 expression in vitro and in vivo. Expression of the truncated form of H-rev107 lacking the COOH-terminal membrane associated domain of 25 amino acids, had a weaker inhibitory effect on proliferation in vitro and was unable to attenuate tumor growth in nude mice. The H-rev107 mRNA is expressed in most adult rat tissues, and immunohistochemical analysis showed expression of the protein in differentiated epithelial cells of stomach, of colon and small intestine, in kidney, bladder, esophagus, and in tracheal and bronchial epithelium. H-rev107 gene transcription is downregulated in rat cell lines derived from liver, kidney, and pancreatic tumors and also in experimental mammary tumors expressing a RAS transgene. In colon carcinoma cell lines only minute amounts of protein were detectable. Thus, downregulation of H-rev107 expression may occur at the level of mRNA or protein.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Breast Neoplasms  ISOPlaat3 (Mus musculus)2290001; 2290001 RGD 
Breast Neoplasms  IDA 2290001mRNA and protein:decreased expression:tumor:mRNA decreased and protein undetectable in HRAS transgene-induced tumorsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Plaat3  (phospholipase A and acyltransferase 3)

Genes (Mus musculus)
Plaat3  (phospholipase A and acyltransferase 3)

Genes (Homo sapiens)
PLAAT3  (phospholipase A and acyltransferase 3)


Additional Information