RGD Reference Report - Differential expression of E-cadherin and beta catenin in primary and metastatic Wilms's tumours. - Rat Genome Database

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Differential expression of E-cadherin and beta catenin in primary and metastatic Wilms's tumours.

Authors: Alami, J  Williams, BR  Yeger, H 
Citation: Alami J, etal., Mol Pathol. 2003 Aug;56(4):218-25.
RGD ID: 2289833
Pubmed: PMID:12890743   (View Abstract at PubMed)
PMCID: PMC1187324   (View Article at PubMed Central)

BACKGROUND: The E-cadherin-catenin adhesion complex is crucial for intercellular adhesiveness and maintenance of tissue architecture. Its impairment is associated with poorly differentiated phenotype and increased invasiveness of carcinomas. AIMS: To evaluate E-cadherin, beta catenin, gamma catenin, and ezrin expression and its relation to histopathological features of primary and metastatic Wilms's tumours. METHODS: Immunohistochemistry was used to determine the expression and cellular distribution of E-cadherin, beta catenin, gamma catenin, and ezrin in primary and metastatic Wilms's tumours. Western blotting was used to determine polypeptide size and expression of E-cadherin and beta catenin in Wilms's tumours compared with normal kidney. RESULTS: Moderate expression of E-cadherin was found mainly in cytoplasm and occasionally cell membranes of dysplastic tubules, whereas low expression was seen in cytoplasm of blastemal cells. Primary and metastatic tumours showed moderate to high beta catenin expression in blastemal and epithelial cells, with predominantly membranous and cytoplasmic staining. Occasional nuclear staining was noted in metastatic tumours. Low to high gamma catenin and ezrin expression was seen in cytoplasm of blastemal and epithelial cells of primary and metastatic tumours. Higher amounts of 92 kDa beta catenin were detected in tumours than in normal kidney. Low expression of 120 kDa E-cadherin was seen in moderately differentiated tumours, whereas expression was lacking in poorly differentiated tumours. CONCLUSIONS: Compared with primary tumours, metastatic tumours showed lower expression of E-cadherin and gamma catenin, with nuclear staining for beta catenin. Low E-cadherin was associated with poorly differentiated tumours. These results suggest that abnormal expression of adhesion proteins correlates with the invasive and metastatic phenotype in Wilms's tumours.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nephroblastoma  IEP 2289833protein:increased expression:kidneyRGD 
nephroblastoma  ISOCTNNB1 (Homo sapiens)2289833; 2289833protein:increased expression:kidneyRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ctnnb1  (catenin beta 1)

Genes (Mus musculus)
Ctnnb1  (catenin beta 1)

Genes (Homo sapiens)
CTNNB1  (catenin beta 1)


Additional Information