RGD Reference Report - Induction of cytosolic aspartate aminotransferase by glucagon in primary cultured rat hepatocytes. - Rat Genome Database

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Induction of cytosolic aspartate aminotransferase by glucagon in primary cultured rat hepatocytes.

Authors: Horio, Y  Fukui, H  Taketoshi, M  Tanaka, T  Wada, H 
Citation: Horio Y, etal., Biochem Biophys Res Commun. 1988 May 31;153(1):410-6.
RGD ID: 2289377
Pubmed: PMID:2837211   (View Abstract at PubMed)

The activity and the mRNA content of cytosolic aspartate aminotransferase (EC 2.6.1.1) were examined in cultured rat hepatocytes. Addition of glucagon (1 x 10(-7) M) in the presence of dexamethasone (1 x 10(-7) M) caused about 2-fold increase in the activity and mRNA content. Dibutyryl cAMP (1 x 10(-4) M) could replace glucagon for this effect. Maximal induction of cytosolic aspartate aminotransferase mRNA was observed 8 h after their additions. Insulin (1 x 10(-7) M) did not inhibit the enzyme induction by glucagon or dibutyryl cAMP. These results suggest that the cytosolic aspartate aminotransferase gene is regulated by cAMP, and not by insulin.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Got1Rataspartate metabolic process  IDA  RGD 
Got1Ratdicarboxylic acid metabolic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Got1Ratcarboxylic acid binding  IDA  RGD 
Got1RatL-aspartate:2-oxoglutarate aminotransferase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Got1  (glutamic-oxaloacetic transaminase 1)


Additional Information