RGD Reference Report - Gene profiling in Pap-cell smears of high-risk human papillomavirus-positive squamous cervical carcinoma. - Rat Genome Database

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Gene profiling in Pap-cell smears of high-risk human papillomavirus-positive squamous cervical carcinoma.

Authors: Manavi, M  Hudelist, G  Fink-Retter, A  Gschwandtler-Kaulich, D  Pischinger, K  Czerwenka, K 
Citation: Manavi M, etal., Gynecol Oncol. 2007 May;105(2):418-26. Epub 2007 Feb 15.
RGD ID: 2289084
Pubmed: PMID:17306351   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ygyno.2006.12.032   (Journal Full-text)

OBJECTIVE: The purpose of the study was to investigate benign and malignant squamous cervical cells obtained by cervical swabs with regard to differentially expressed genes and gene expression profiling, in order to evaluate the biological behavior and clinical outcome of cervical malignancies. METHODS: Cervical squamous cells from six women with high-risk human papillomavirus positive [HR-HPV(+)] cervical carcinoma and from six HPV-negative women with normal ectocervical cells were analyzed by cDNA array. RESULTS: cDNA over-expression of several genes such as MET (c-met), Nm23-H1 (NME1), EGFR, KGFR, Nm23-H2 (NME2), ERBB2 (c-erbB-2), cyclin-dependent kinase inhibitor 4 (CDKN2A, p16INK4A), cytokeratin 8 (KRT8), KRAS (K-ras), FLT1, KGF (FGF7), BCL2-like 2 protein (BCL2L2), ERBB4, MYCN (N-myc), cyclin D1 (CCND1), KIT (c-kit), secreted phosphoprotein 1 (SPP1) and STAT1, was significant in cervical squamous cell carcinoma (CSCC). Gene expression was downregulated for 13 genes in CSCC, such as interleukin 1 alpha (IL1A), the transforming growth factor receptor beta superfamily (TGFbeta; TGFB), some members of the insulin-like growth factor binding proteins (IGFBPs) and the integrin family (ITGA6, ITGB1). CONCLUSION: This study was focused on the gene expression profiling of HR-HPV(-) and (+) cervical squamous cells and CSCC obtained by cytobrush. We observed gene expression patterns and signaling pathways that permit the investigator to distinguish between benign squamous cervical cells and CSCC with and without HPV infection.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cervical squamous cell carcinoma  IEP 2289084cervical squamous cell carcinoma and mRNA:increased expression:cervical squamous cellsRGD 
cervical squamous cell carcinoma  ISOFGF7 (Homo sapiens)2289084; 2289084cervical squamous cell carcinomaRGD 
squamous cell carcinoma  IEP 2289084; 2289084cervical squamous cell carcinoma and mRNA:increased expression:cervical squamous cellsRGD 
squamous cell carcinoma  ISOFGF7 (Homo sapiens)2289084; 2289084cervical squamous cell carcinomaRGD 
squamous cell carcinoma  ISOFGFR2 (Homo sapiens)2289084; 2289084cervical squamous cell carcinomaRGD 
Uterine Cervical Neoplasms  IEP 2289084cervical squamous cell carcinoma and mRNA:increased expression:cervical squamous cellsRGD 
Uterine Cervical Neoplasms  IEP 2289084mRNA:increased expression:uterine cervixRGD 
Uterine Cervical Neoplasms  ISOFGFR2 (Homo sapiens)2289084; 2289084cervical squamous cell carcinomaRGD 
Uterine Cervical Neoplasms  ISOFLT1 (Homo sapiens)2289084; 2289084mRNA:increased expression:uterine cervixRGD 

Objects Annotated

Genes (Rattus norvegicus)
Fgf7  (fibroblast growth factor 7)
Fgfr2  (fibroblast growth factor receptor 2)
Flt1  (Fms related receptor tyrosine kinase 1)

Genes (Mus musculus)
Fgf7  (fibroblast growth factor 7)
Fgfr2  (fibroblast growth factor receptor 2)
Flt1  (FMS-like tyrosine kinase 1)

Genes (Homo sapiens)
FGF7  (fibroblast growth factor 7)
FGFR2  (fibroblast growth factor receptor 2)
FLT1  (fms related receptor tyrosine kinase 1)


Additional Information