RGD Reference Report - BTG2(TIS21/PC3) induces neuronal differentiation and prevents apoptosis of terminally differentiated PC12 cells. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

BTG2(TIS21/PC3) induces neuronal differentiation and prevents apoptosis of terminally differentiated PC12 cells.

Authors: El-Ghissassi, F  Valsesia-Wittmann, S  Falette, N  Duriez, C  Walden, PD  Puisieux, A 
Citation: el-Ghissassi F, etal., Oncogene. 2002 Oct 3;21(44):6772-78.
RGD ID: 2289077
Pubmed: PMID:12360398   (View Abstract at PubMed)
DOI: DOI:10.1038/sj.onc.1205888   (Journal Full-text)

The p53-transcriptional target, BTG2(TIS21/PC3), was previously identified as an antiproliferative gene. However, the precise biological functions of the protein product remain to be elucidated. BTG2(TIS21/PC3) expression is induced in vivo during neurogenesis, and the gene is transiently expressed in vitro in rat pheochromocytoma PC12 cells after induction of neuronal differentiation by addition of nerve growth factor (NGF). These observations suggest that BTG2(TIS21/PC3) is functionally significant during the neuronal differentiation process. To test this hypothesis, a vector that expressed BTG2(TIS21/PC3) under the control of an inducible promoter was introduced into PC12 cells. Growth arrest and differentiation in response to NGF were greatly enhanced by BTG2(TIS21/PC3) overexpression. Furthermore, an antisense oligonucleotide complementary to BTG2(TIS21/PC3) mRNA, which was able to inhibit endogenous BTG2(TIS21/PC3) expression, triggered programmed cell death in differentiated PC12 cells. These observations confirm that BTG2(TIS21/PC3) expression promotes neuronal differentiation and that it is required for survival of terminally differentiated cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of apoptotic process acts_upstream_of_or_withinIDA 2289077PMID:12360398MGI 
negative regulation of neuron apoptotic process  IMP 2289077 RGD 
neuron differentiation acts_upstream_of_or_withinIDA 2289077PMID:12360398MGI 

Objects Annotated

Genes (Rattus norvegicus)
Btg2  (BTG anti-proliferation factor 2)


Additional Information