RGD Reference Report - Assembly of Taurine Transporter (Slc6a6) with Na+-H+ Exchanger Regulatory Factor 1 (Slc9a3r1) Improves GABA Transport Activity by Increasing the Maximum Transport Velocity. - Rat Genome Database

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Assembly of Taurine Transporter (Slc6a6) with Na+-H+ Exchanger Regulatory Factor 1 (Slc9a3r1) Improves GABA Transport Activity by Increasing the Maximum Transport Velocity.

Authors: Tachikawa, Masanori  Yokoyama, Reiji  Akanuma, Shin-Ichi  Hosoya, Ken-Ichi 
Citation: Tachikawa M, etal., Biol Pharm Bull. 2018;41(3):338-341. doi: 10.1248/bpb.b17-00664.
RGD ID: 21201269
Pubmed: PMID:29491210   (View Abstract at PubMed)
DOI: DOI:10.1248/bpb.b17-00664   (Journal Full-text)

Regulating γ-aminobutyric acid (GABA) uptake transport on the plasma membranes is required for its efficient clearance from the brain interstitial fluid. The purpose of this study was to clarify the assembly of taurine transporter (TauT/Slc6a6) and PSD-95/Disc-large/Zo-1 (PDZ) domain of Na+-H+ exchanger regulatory factor 1 (NHERF1) as a regulatory mechanism of TauT-mediated GABA transport activity. In vitro glutathione S-transferase (GST)-pull down assay and immunoblotting with anti-NHERF1 antibody revealed that NHERF1 protein was present in rat brain lysates as the binding protein of the GST-fusion TauT C-terminal protein with the PDZ-binding ETMM motif but not its corresponding deletion mutant lacking the motif. [3H]GABA uptake by TauT-NHERF1-coexpressing oocytes and TauT-singly expressing oocytes exhibited saturable kinetics with Michaelis-Menten constant values of 0.835±0.288 and 0.982±0.569 mM and a maximal transport velocity of 206±37 and 283±28 pmol/(h·oocyte), respectively. These results suggest that the assembly of TauT PDZ-binding motif and NHERF1 increases the maximal transport velocity of GABA rather than changes the affinity.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
plasma membrane protein complex is_active_inIPIUniProtKB:P3164321201269PMID:29491210ARUK-UCL 
plasma membrane protein complex is_active_inIPIUniProtKB:Q9JJ1921201269PMID:29491210ARUK-UCL 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Nherf1  (NHERF family PDZ scaffold protein 1)
Slc6a6  (solute carrier family 6 member 6)


Additional Information