RGD Reference Report - Smad4 expression in hepatocellular carcinoma differs by hepatitis status. - Rat Genome Database

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Smad4 expression in hepatocellular carcinoma differs by hepatitis status.

Authors: Yao, Lei  Li, Fu-Jun  Tang, Zhi-Qiang  Gao, Shuang  Wu, Qe-Quan 
Citation: Yao L, etal., Asian Pac J Cancer Prev. 2012;13(4):1297-303. doi: 10.7314/apjcp.2012.13.4.1297.
RGD ID: 21066341
Pubmed: PMID:22799322   (View Abstract at PubMed)
DOI: DOI:10.7314/apjcp.2012.13.4.1297   (Journal Full-text)


AIMS: Primary hepatocellular carcinoma (HCC) is a common malignancy often related to hepatitis viral infection. Smad4 is known to mediate the TGF-β pathway to suppress tumorigenesis. However, the function of Smad4 in HCC is still controversial. In this study we compared levels of Smad4 in HCC tissues with or without hepatitis virus infection and adjacent normal-appearing liver.
METHODS: Samples from HCC patients were analyzed for Smad4 protein and mRNA expression by immunohistochemistry (IHC), RT-PCR and Western blotting.
RESULTS: We found that tumor tissues expressed less Smad4 mRNA and protein than the adjacent tissues. Most HCC tumor tissues were negative for Smad4 in IHC staining, while the majority of adjacent tissues were positively stained. Interestingly, protein levels were higher in HCC tissues with viral hepatitis than those without virus infection. Suppression of expression appeared closely related to HCC, so that Smad4 appears to function as a tumor suppressor gene (TSG).
CONCLUSION: Patients with hepatitis viral infection, at higher risk for HCC, exhibited increased Smad4 protein expression suggesting hepatitis virus may modulate Smad4 expression, which is functionally distinct from its putative role as a TSG. Smad4 expression may thus be an applicable marker for diagnosis and/or a target to develop therapeutic agents for HCC.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SMAD4Humanhepatocellular carcinoma  IEP protein:decreased expression:liverRGD 
Smad4Rathepatocellular carcinoma  ISOSMAD4 (Homo sapiens)protein:decreased expression:liverRGD 
Smad4Mousehepatocellular carcinoma  ISOSMAD4 (Homo sapiens)protein:decreased expression:liverRGD 
SMAD4HumanHuman Viral Hepatitis  IEP associated with hepatocellular carcinoma and protein:altered expression:liverRGD 
Smad4RatHuman Viral Hepatitis  ISOSMAD4 (Homo sapiens)associated with hepatocellular carcinoma and protein:altered expression:liverRGD 
Smad4MouseHuman Viral Hepatitis  ISOSMAD4 (Homo sapiens)associated with hepatocellular carcinoma and protein:altered expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Smad4  (SMAD family member 4)

Genes (Mus musculus)
Smad4  (SMAD family member 4)

Genes (Homo sapiens)
SMAD4  (SMAD family member 4)


Additional Information