RGD Reference Report - Transcriptome clarifies mechanisms of lesion genesis versus progression in models of Ccm3 cerebral cavernous malformations. - Rat Genome Database

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Transcriptome clarifies mechanisms of lesion genesis versus progression in models of Ccm3 cerebral cavernous malformations.

Authors: Koskimäki, Janne  Zhang, Dongdong  Li, Yan  Saadat, Laleh  Moore, Thomas  Lightle, Rhonda  Polster, Sean P  Carrión-Penagos, Julián  Lyne, Seán B  Zeineddine, Hussein A  Shi, Changbin  Shenkar, Robert  Romanos, Sharbel  Avner, Kenneth  Srinath, Abhinav  Shen, Le  Detter, Matthew R  Snellings, Daniel  Cao, Ying  Lopez-Ramirez, Miguel A  Fonseca, Gregory  Tang, Alan T  Faber, Pieter  Andrade, Jorge  Ginsberg, Mark  Kahn, Mark L  Marchuk, Douglas A  Girard, Romuald  Awad, Issam A 
Citation: Koskimäki J, etal., Acta Neuropathol Commun. 2019 Aug 19;7(1):132. doi: 10.1186/s40478-019-0789-0.
RGD ID: 18899564
Pubmed: (View Article at PubMed) PMID:31426861
DOI: Full-text: DOI:10.1186/s40478-019-0789-0

Cerebral cavernous malformations (CCMs) are dilated capillaries causing epilepsy and stroke. Inheritance of a heterozygous mutation in CCM3/PDCD10 is responsible for the most aggressive familial form of the disease. Here we studied the differences and commonalities between the transcriptomes of microdissected lesional neurovascular units (NVUs) from acute and chronic in vivo Ccm3/Pdcd10ECKO mice, and cultured brain microvascular endothelial cells (BMECs) Ccm3/Pdcd10ECKO.We identified 2409 differentially expressed genes (DEGs) in acute and 2962 in chronic in vivo NVUs compared to microdissected brain capillaries, as well as 121 in in vitro BMECs with and without Ccm3/Pdcd10 loss (fold change >= |2.0|; p < 0.05, false discovery rate corrected). A functional clustered dendrogram generated using the Euclidean distance showed that the DEGs identified only in acute in vivo NVUs were clustered in cellular proliferation gene ontology functions. The DEGs only identified in chronic in vivo NVUs were clustered in inflammation and immune response, permeability, and adhesion functions. In addition, 1225 DEGs were only identified in the in vivo NVUs but not in vitro BMECs, and these clustered within neuronal and glial functions. One miRNA mmu-miR-3472a was differentially expressed (FC = - 5.98; p = 0.07, FDR corrected) in the serum of Ccm3/Pdcd10+/- when compared to wild type mice, and this was functionally related as a putative target to Cand2 (cullin associated and neddylation dissociated 2), a DEG in acute and chronic lesional NVUs and in vitro BMECs. Our results suggest that the acute model is characterized by cell proliferation, while the chronic model showed inflammatory, adhesion and permeability processes. In addition, we highlight the importance of extra-endothelial structures in CCM disease, and potential role of circulating miRNAs as biomarkers of disease, interacting with DEGs. The extensive DEGs library of each model will serve as a validation tool for potential mechanistic, biomarker, and therapeutic targets.

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Cand2  (cullin-associated and neddylation-dissociated 2 (putative))

Genes (Mus musculus)
Cand2  (cullin-associated and neddylation-dissociated 2 (putative))

Genes (Homo sapiens)
CAND2  (cullin associated and neddylation dissociated 2 (putative))

Additional Information