RGD Reference Report - Immunolocalization of metabotropic glutamate receptor 7 in the rat olfactory bulb. - Rat Genome Database

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Immunolocalization of metabotropic glutamate receptor 7 in the rat olfactory bulb.

Authors: Kinzie, JM  Shinohara, MM  Van den Pol, AN  Westbrook, GL  Segerson, TP 
Citation: Kinzie JM, etal., J Comp Neurol. 1997 Sep 1;385(3):372-84.
RGD ID: 1643599
Pubmed: PMID:9300765   (View Abstract at PubMed)

Metabotropic glutamate receptors (mGluRs) constitute a large family of G-protein-coupled receptors that are subdivided into three groups based on sequence similarity, pharmacological profiles, and coupling to second messengers. Although mRNAs for seven of the eight mGluRs are expressed in the olfactory system, the localization and function of specific subtypes have not been fully characterized. Mitral cells of the olfactory bulb express mRNA for several mGluRs, including mGluR7, which has been suggested as a presynaptic glutamate autoreceptor. To investigate the immunolocalization of mGluR7 in the olfactory system, we used a polyclonal antiserum specific for the carboxy terminus of the receptor. Mitral cell somata and proximal dendrites were strongly labeled by the mGluR7 antibody. Electron microscopic analysis revealed that most of the mitral cell somatic staining was cytoplasmic. In olfactory bulb glomeruli, immunoreactivity was present in axons and dendrites. In the piriform cortex, diffuse staining was present in layer Ia that was markedly reduced following bulbectomy, consistent with expression of mGluR7 in mitral cell axon terminals. Electron microscopic analysis of this region confirmed the presence of mGluR7 in multiple axon terminals. Distinct labeled fibers in all levels of layer I appeared to originate from labeled piriform cortex pyramidal cells in layers II and III. Our results indicate that mGluR7 is primarily presynaptic at olfactory bulb synapses. However, the postsynaptic localization of mGluR7 at selected synapses indicates that mGluR7 is not targeted exclusively to axonal compartments.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
asymmetric synapse  IDA 1643599 RGD 
axon  IDA 1643599 RGD 
dendrite  IDA 1643599 RGD 
neuronal cell body  IDA 1643599 RGD 
presynaptic active zone  IDA 1643599 RGD 
presynaptic membrane  IDA 1643599 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Grm7  (glutamate metabotropic receptor 7)


Additional Information