RGD Reference Report - A high soy diet reduces programmed cell death and enhances bcl-xL expression in experimental stroke. - Rat Genome Database

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A high soy diet reduces programmed cell death and enhances bcl-xL expression in experimental stroke.

Authors: Lovekamp-Swan, T  Glendenning, M  Schreihofer, DA 
Citation: Lovekamp-Swan T, etal., Neuroscience. 2007 Sep 7;148(3):644-52. Epub 2007 Jul 17.
RGD ID: 1643491
Pubmed: PMID:17706879   (View Abstract at PubMed)
PMCID: PMC2788970   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neuroscience.2007.06.046   (Journal Full-text)

Soy phytoestrogens have been proposed as an alternative to estrogen replacement therapy and have demonstrated potential neuroprotective effects in the brain. We have shown that a high soy diet significantly reduces infarct size following permanent middle cerebral artery occlusion (MCAO). Here, we tested the hypothesis that a high soy diet would attenuate programmed cell death after stroke. Adult female Sprague-Dawley rats were ovariectomized and fed either an isoflavone-reduced diet (IFP) or a high soy diet (SP) for 2 weeks before undergoing 90 min of transient middle cerebral artery occlusion (tMCAO) followed by 22.5 h reperfusion. Infarct size, as assessed by triphenyltetrazolium chloride staining, was significantly reduced by a high soy diet (P<0.05). Apoptosis in the ischemic cortex, measured by TUNEL staining, was significantly reduced by the high soy diet. The number of active caspase-3 positive cells and caspase-mediated alpha-spectrin cleavage were also significantly decreased in the ischemic cortex of SP rats. Furthermore, nuclear translocation of apoptosis-inducing factor (AIF) was significantly reduced in the ischemic cortex of SP rats. Soy significantly increased bcl-x(L) mRNA and protein expression in the ischemic cortex compared with IFP rats. Immunohistochemistry revealed increased neuronal expression of bcl-2 and bcl-x(L) in the ischemic cortex of both IFP and SP rats following tMCAO. These results suggest that a high soy diet decreases both caspase-dependent and caspase-independent programmed cell death following tMCAO. Further, a high soy diet enhances expression of the cell survival factor bcl-x(L) following tMCAO, contributing to the neuroprotective effects of soy in the ischemic cortex.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
brain ischemia  ISOBcl2l1 (Rattus norvegicus)1643491; 1643491protein:increased expression:cerebral cortexRGD 
brain ischemia  IEP 1643491protein:increased expression:cerebral cortexRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bcl2l1  (Bcl2-like 1)

Genes (Mus musculus)
Bcl2l1  (BCL2-like 1)

Genes (Homo sapiens)
BCL2L1  (BCL2 like 1)


Additional Information