RGD Reference Report - Protein kinase C regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression during rat Sertoli cell development. - Rat Genome Database
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Protein kinase C regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression during rat Sertoli cell development.

Authors: Brucato, S  Villers, C 
Citation: Brucato S and Villers C, Biochimie. 2002 Jul;84(7):681-6.
RGD ID: 1643137
Pubmed: (View Article at PubMed) PMID:12453641

Our previous studies indicated that cell surface proteoglycans were mostly heparan sulfate ones (HSPG) in 20 day-old Sertoli cells [Biochim. Biophys. Acta 1510 (2001) 474]. Among these HSPG, glypican-1, syndecans-1 and -4 mRNAs were expressed and differentially regulated. Glypican-1 and syndecan-1 mRNA expression was up-regulated under PKC activation in contrast to syndecan-4 mRNA expression which was not affected [Biochim. Biophys. Acta 1474 (2000) 31]. Rat Sertoli cells undergo extensive changes during the postnatal period both in structure and function, as the hematotesticular barrier establishment occurs at around 20 day-old. The testicular PKCalpha expression in developing Sertoli cells results in (i) a soluble (inactive) form which is maximal at the age of 1 day and declines gradually thereafter and (ii) a particulate (active) form which is low at birth, increases six-fold on days 8-11 of age and declines thereafter. The present study focused on the glypican-1, syndecan-1 and syndecan-4 mRNA expression and regulation under PKC activation by the phorbol myristate acetate (PMA) in 10-30 day-old Sertoli cells. Our data indicated that the regulation of their expression specifically depends on the nature of HSPG and Sertoli cell developmental stage and evidenced a specific PKC regulation of HSPG mRNA expression.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Sdc1  (syndecan 1)


Additional Information