RGD Reference Report - Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum. - Rat Genome Database

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Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum.

Authors: Lees, SJ  Chen, YT  Williams, JH 
Citation: Lees SJ, etal., Acta Physiol Scand. 2004 Jun;181(2):239-45.
RGD ID: 1642741
Pubmed: PMID:15180797   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1365-201X.2004.01278.x   (Journal Full-text)

AIMS: Gel electrophoresis revealed a band of molecular weight approximately 160 000 Da associated with the skeletal muscle sarcoplasmic reticulum (SR) vesicle preparations. This investigation sought to examine glycogen debranching enzyme associated with skeletal muscle SR. METHODS: Sarcoplasmic reticulum samples were also taken from muscle whose glycogen content had been reduced either via stimulation of the sciatic nerve or alpha-amylase treatment of muscle homogenates. RESULTS: The stimulation protocol reduced whole muscle glycogen by 86% (7.4 +/- 0.4 vs. 1.0 +/- 0.3 microg mg(-1) wet mass, P < or = 0.05). Glycogen associated with the SR was reduced by 82% in the stimulation protocol (533 +/- 82 vs. 96 +/- 7 microg mg(-1) protein) and by 94% in alpha-amylase treatment (493 +/- 11 vs. 29 +/- 2 microg mg(-1) protein), respectively. Gel electrophoresis and Western blots revealed that the content of glycogen debranching enzyme was reduced by approximately 53% as a result of muscle stimulation and by approximately 46% in alpha-amylase treatment (P < or = 0.05). In addition, glycogen debranching enzyme activity was reduced by 61% in stimulated samples compared with control (20.3 +/- 1.0 vs. 8.0 +/- 1.2 nmol mg(-1) min(-1), respectively), a value consistent with reductions observed from gel electrophoresis and Western blots. CONCLUSION: These results confirm that similar to glycogen phosphorylase, glycogen debranching enzyme is associated with the skeletal muscle SR and is dissociated under exercise conditions.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AglRatglycogen catabolic process  IDA  RGD 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AglRatsarcoplasmic reticulum  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AglRat4-alpha-glucanotransferase activity  IDA  RGD 
AglRatamylo-alpha-1,6-glucosidase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AGLHumanglycogen degradation pathway   ISOAgl (Rattus norvegicus) RGD 
AglRatglycogen degradation pathway   IDA  RGD 
AglMouseglycogen degradation pathway   ISOAgl (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Agl  (amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase)

Genes (Mus musculus)
Agl  (amylo-1,6-glucosidase, 4-alpha-glucanotransferase)

Genes (Homo sapiens)
AGL  (amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase)

Additional Information