RGD Reference Report - The stoichiometry of P2X2/6 receptor heteromers depends on relative subunit expression levels. - Rat Genome Database

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The stoichiometry of P2X2/6 receptor heteromers depends on relative subunit expression levels.

Authors: Barrera, NP  Henderson, RM  Murrell-Lagnado, RD  Edwardson, JM 
Citation: Barrera NP, etal., Biophys J. 2007 Jul 15;93(2):505-12. Epub 2007 Apr 20.
RGD ID: 1642655
Pubmed: PMID:17449665   (View Abstract at PubMed)
PMCID: PMC1896263   (View Article at PubMed Central)
DOI: DOI:10.1529/biophysj.106.101048   (Journal Full-text)

Fast synaptic transmission involves the operation of ionotropic receptors, which are often composed of at least two types of subunit. We have developed a method, based on atomic force microscopy imaging to determine the stoichiometry and subunit arrangement within ionotropic receptors. We showed recently that the P2X(2) receptor for ATP is expressed as a trimer but that the P2X(6) subunit is unable to oligomerize. In this study we addressed the subunit stoichiometry of heteromers containing both P2X(2) and P2X(6) subunits. We transfected tsA 201 cells with both P2X(2) and P2X(6) subunits, bearing different epitope tags. We manipulated the transfection conditions so that either P2X(2) or P2X(6) was the predominant subunit expressed. By atomic force microscopy imaging of isolated receptors decorated with antiepitope antibodies, we demonstrate that when expression of the P2X(2) subunit predominates, the receptors contain primarily 2 x P2X(2) subunits and 1 x P2X(6) subunit. In contrast, when the P2X(6) subunit predominates, the subunit stoichiometry of the receptors is reversed. Our results show that the composition of P2X receptor heteromers is plastic and dependent on the relative subunit expression levels. We suggest that this property of receptor assembly might introduce an additional layer of subtlety into P2X receptor signaling.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2rx2Ratprotein-containing complex binding  IDA heterooligomerizationRGD 
P2rx6Ratprotein-containing complex binding  IDA heterooligomerizationRGD 

Objects Annotated

Genes (Rattus norvegicus)
P2rx2  (purinergic receptor P2X 2)
P2rx6  (purinergic receptor P2X 6)

Additional Information