RGD Reference Report - Congenic Interval Mapping of RNO10 Reveals a Complex Cluster of Closely-Linked Genetic Determinants of Blood Pressure. - Rat Genome Database

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Congenic Interval Mapping of RNO10 Reveals a Complex Cluster of Closely-Linked Genetic Determinants of Blood Pressure.

Authors: Saad, Y  Yerga-Woolwine, S  Saikumar, J  Farms, P  Manickavasagam, E  Toland, EJ  Joe, B 
Citation: Saad Y, etal., Hypertension. 2007 Sep 24;.
RGD ID: 1642602
Pubmed: (View Article at PubMed) PMID:17893371
DOI: Full-text: DOI:10.1161/HYPERTENSIONAHA.107.097105

Genetic dissection of the rat genome for identifying alleles that cause abnormalities in blood pressure (BP) resulted in the mapping of a significant number of BP quantitative trait loci (QTLs). In this study we mapped at least one such BP QTL on rat chromosome 10 (RNO10) as being within the introgressed segment of a S.LEW congenic strain S.LEWx12x2x3x8 spanning 1.34 Mb from 70 725 437 bp to 72 063 232 bp. BP of 3 congenic strains that span shorter segments of this region was additionally examined. Results obtained indicate that LEW alleles that comprise a 375-kb introgressed segment of the congenic strain S.LEWx12x2x3x5 (70 725 437 bp to 71 100 513 bp) increase BP. The magnitude of change in BP exhibited by the 2 strains, S.LEWx12x2x3x8 and S.LEWx12x2x3x5, is the net phenotypic effect of the underlying genetic determinants of BP. In this respect, the current data are superior to previous QTL localization of BP QTL1, which was hypothesized based on differential congenic segments. Genetic dissection using these 2 congenic strains as tools is expected to facilitate further dissection of the regions. Meanwhile, differential congenic segments were used to predict and thereby prioritize regions for candidate gene analysis. Using this approach, 2 distinct regions of 401 kb and 409 kb within S.LEWx12x2x3x8 and a 104 kb region within S.LEWx12x2x3x5 were prioritized for further consideration. Because all of these genetic elements are located within a 1.06-Mb region of RNO10, our study has revealed a remarkable insight into a genomic module comprising very closely-linked, opposing genetic determinants of BP.



Disease Annotations    
hypertension  (IAGP,IDA)
Hypotension  (IDA)


Objects Annotated

QTLs
Bp300  (Blood pressure QTL 300)
Bp301  (Blood pressure QTL 301)
Bp302  (Blood pressure QTL 302)

Objects referenced in this article
QTL Bp299 Blood pressure QTL 299 Rattus norvegicus
Strain SS.LEW-(D10Mco84-D10Got93)/Mco null Rattus norvegicus

Additional Information