RGD Reference Report - Neuromedin B-like peptides in rat brain: biochemical characterization, mechanism of release and localization in synaptosomes. - Rat Genome Database

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Neuromedin B-like peptides in rat brain: biochemical characterization, mechanism of release and localization in synaptosomes.

Authors: Moody, TW  Korman, LY  O'Donohue, TL 
Citation: Moody TW, etal., Peptides. 1986 Sep-Oct;7(5):815-20.
RGD ID: 1642349
Pubmed: PMID:3797343   (View Abstract at PubMed)

Neuromedin B-like peptides were characterized in the rat brain. A rabbit antisera was utilized which recognized neuromedin B but not bombesin or GRP. Using gel filtration and HPLC techniques, a major and minor peak of immunoreactivity was present in rat brain extracts. In both cases the main peak of immunoreactivity coeluted with synthetic neuromedin B. The density of neuromedin B-like peptides ranged 50-fold being greatest in the olfactory bulb and hypothalamus, intermediate in the hippocampus, spinal cord, medulla/pons, pituitary, midbrain, thalamus, striatum and cortex and lowest in the cerebellum. Release studies indicated that neuromedin B-like peptides were secreted from hypothalamic, olfactory bulb and thalamic slices in a Ca++-dependent manner when KCl (75 mM) was present. Also, the neuromedin B-like peptides in the rat brain were localized to synaptosomes. These data indicate that neuromedin B-like peptides may function as regulatory peptides in the CNS distinct from bombesin/GRP.

Objects referenced in this article
Gene Nmb neuromedin B Rattus norvegicus

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