RGD Reference Report - Neuromedin B stimulates arachidonic acid release, c-fos gene expression, and the growth of C6 glioma cells. - Rat Genome Database

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Neuromedin B stimulates arachidonic acid release, c-fos gene expression, and the growth of C6 glioma cells.

Authors: Moody, TW  Fagarasan, M  Zia, F 
Citation: Moody TW, etal., Peptides. 1995;16(6):1133-40.
RGD ID: 1642335
Pubmed: PMID:8532598   (View Abstract at PubMed)

The effects of neuromedin B (NMB) on C6 glioma cells were investigated. NMB bound with high affinity (IC50 = 1 nM) to C6 cells whereas BN and GRP were less potent (IC50 = 40 and 100 nM). NMB (1 nM) elevated cytosolic Ca2+ in individual C6 cells and the increase in cytosolic Ca2+ was reversed by 1 microM [D-Arg1, D-Pro2,D-Trp7.9,Leu11]substance P [APTTL]SP, a broad spectrum antagonist. NMB stimulated [3H]arachidonic acid release from C6 cells and the increase in [3H]arachidonic acid release was reversed [APTTL]SP. NMB increased transiently c-fos gene expression in C6 cells. NMB increased the number of C6 colonies in soft agar and the increase in growth caused by NMB was reversed by [APTTL]SP. These data suggest that NMB receptors may regulate the proliferation of C6 cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
arachidonic acid secretion  IDA 1642335stimulatesRGD 
positive regulation of cell population proliferation  IDA 1642335C6 glioma cellsRGD 
positive regulation of cytosolic calcium ion concentration  IDA 1642335 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nmb  (neuromedin B)


Additional Information