RGD Reference Report - Glucokinase regulatory protein is associated with mitochondria in hepatocytes. - Rat Genome Database

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Glucokinase regulatory protein is associated with mitochondria in hepatocytes.

Authors: Arden, C  Baltrusch, S  Agius, L 
Citation: Arden C, etal., FEBS Lett. 2006 Apr 3;580(8):2065-70. Epub 2006 Mar 10.
RGD ID: 1626614
Pubmed: PMID:16542652   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2006.03.009   (Journal Full-text)

The association of glucokinase with liver mitochondria has been reported [Danial et al. (2003) BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424, 952-956]. We confirmed association of glucokinase immunoreactivity with rat liver mitochondria using Percoll gradient centrifugation and demonstrated its association with the 68 kDa regulatory protein (GKRP) but not with the binding protein phosphofructokinase-2/fructose bisphosphatase-2. Substrates and glucagon induced adaptive changes in the mitochondrial glucokinase/GKRP ratio suggesting a regulatory role for GKRP. Combined with previous observations that GKRP overexpression partially inhibits glycolysis [de la Iglesia et al. (2000) The role of the regulatory protein of glucokinase in the glucose sensory mechanism of the hepatocyte. J. Biol. Chem. 275, 10597-10603] these findings suggest that there may be distinct glycolytic pools of glucokinase.

Objects referenced in this article
Gene Gck glucokinase Rattus norvegicus
Gene Gckr glucokinase regulator Rattus norvegicus

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