RGD Reference Report - Differential expression of egr-1 in osteoarthritic compared to normal adult human articular cartilage. - Rat Genome Database

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Differential expression of egr-1 in osteoarthritic compared to normal adult human articular cartilage.

Authors: Wang, FL  Connor, JR  Dodds, RA  James, IE  Kumar, S  Zou, C  Lark, MW  Gowen, M  Nuttall, ME 
Citation: Wang FL, etal., Osteoarthritis Cartilage. 2000 May;8(3):161-9.
RGD ID: 1626498
Pubmed: PMID:10806043   (View Abstract at PubMed)
DOI: DOI:10.1053/joca.1999.0295   (Journal Full-text)

OBJECTIVE: The purpose of this study was to identify genes that are differentially expressed in normal versus osteoarthritic human articular cartilage as either potential novel therapeutic targets or diagnostic markers of this disease. DESIGN: mRNA was isolated from histologically normal and osteoarthritic adult human articular cartilage. The Differential Display technique was employed which identified differentially expressed genes in the normal and diseased tissue. Northern and reverse Northern hybridization were used to confirm the gene expression pattern. Immunohistochemistry and in-situ hybridization were used to localize expression of Egr-1 protein and mRNA respectively in cartilage. RESULTS: A transcription factor, early growth response protein-1 (Egr-1) was found to be down-regulated more than six-fold in multiple human OA cartilage samples when compared to normal tissue. Immunohistochemistry indicated that Egr-1 was expressed throughout normal adult cartilage, in deep-, mid- and superficial-zones. In contrast, in OA cartilage there was expression of Egr-1 mRNA and protein only in the chondrocytes undergoing cloning. CONCLUSIONS: Egr-1 is differentially expressed in OA versus normal cartilage and because of its role in transcriptional activation and repression and regulation of proliferation, differentiation and apoptosis, Egr-1 may play an important role in the pathogenesis of OA. Up-regulation of Egr-1 may therefore provide a novel therapeutic approach for either the prevention or treatment of OA.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
osteoarthritis  IEP 1626498mRNA and protein: decreased expressionRGD 
osteoarthritis  ISOEGR1 (Homo sapiens)1626498; 1626498 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Egr1  (early growth response 1)

Genes (Mus musculus)
Egr1  (early growth response 1)

Genes (Homo sapiens)
EGR1  (early growth response 1)


Additional Information