Submit Data |  Help |  Video Tutorials |  News |  Publications |  FTP Download |  REST API |  Citing RGD |  Contact   

Lysophosphatidic acid stimulates cell proliferation in rat chondrocytes.

Authors: Kim, MK  Lee, HY  Park, KS  Shin, EH  Jo, SH  Yun, J  Lee, SW  Yoo, YH  Lee, YS  Baek, SH  Bae, YS 
Citation: Kim MK, etal., Biochem Pharmacol. 2005 Dec 5;70(12):1764-71. Epub 2005 Oct 20.
Pubmed: (View Article at PubMed) PMID:16242672
DOI: Full-text: DOI:10.1016/j.bcp.2005.09.015

Rat primary chondrocytes express the lysophosphatidic acid (LPA) receptor, LPA1, LPA3, but not LPA2. When chondrocytes were stimulated with LPA, phospholipase C-mediated cytosolic calcium increase was dramatically induced. LPA also stimulated two kinds of mitogen-activated protein kinase, extracellular signal-regulated kinase (ERK) and p38 kinase in chondrocytes. In terms of the LPA-mediated functional modulation of chondrocytes, LPA stimulated cellular proliferation. We examined the signaling pathways involved in LPA-mediated cellular proliferation. LPA-induced chondrocyte proliferation was almost completely blocked by 2'-amino-3'-methoxyflavone (PD98059) but not by 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole (SB203580), suggesting that ERK activity is essentially required for the process. Pertussis toxin almost completely inhibited the LPA-induced cellular proliferation and ERK activation, indicating the role of G(i/o) protein(s) in the processes. This study demonstrates the physiological role of LPA on the modulation of rat primary chondrocyte proliferation, and the crucial role played by ERK in the process.

Annotation

Gene Ontology Annotations
Molecular Pathway Annotations
Objects Annotated

Additional Information

 
RGD Object Information
RGD ID: 1626474
Created: 2007-08-09
Species: All species
Last Modified: 2007-08-09
Status: ACTIVE



NHLBI Logo

RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.