RGD Reference Report - Testosterone modulates Ca(v2.2) calcium channels' functional expression at rat levator ani neuromuscular junction. - Rat Genome Database

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Testosterone modulates Ca(v2.2) calcium channels' functional expression at rat levator ani neuromuscular junction.

Authors: Nudler, SI  Pagani, MR  Urbano, FJ  McEnery, MW  Uchitel, OD 
Citation: Nudler SI, etal., Neuroscience. 2005;134(3):817-26.
RGD ID: 1626316
Pubmed: (View Article at PubMed) PMID:15987667
DOI: Full-text: DOI:10.1016/j.neuroscience.2005.03.061

Spinal nucleus of bulbocavernosus and its target musculature, the bulbocavernosus and levator ani muscles, are sexually dimorphic, and their sexual differentiation depends on plasmatic levels of testosterone. Electrophysiological and immunocytochemical studies have demonstrated that at mammalian adult neuromuscular junctions only P/Q-type Ca2+ channels (Ca(v2.1)), mediate evoked transmitter release. Here we report that N-type Ca2+ channel (Ca(v2.2)) blocker omega-Conotoxin GVIA, as well as Ca(v2.1) blocker omega-Agatoxin IVA, significantly reduced quantal content of transmitter release by approximately 80% and approximately 70% respectively at levator ani muscle of the adult rats, indicating that neuromuscular transmission is jointly mediated by both types of channels. In these synapses, we also observed that castration and restitution of plasmatic testosterone in rats resulted in changes in the sensitivity to omega-Conotoxin GVIA. Castration induced, whereas testosterone treatment avoided, functional loss of Ca(v2.2), as mediators of transmitter release in these synapses. Strikingly, the expression and localization of alpha1B subunits, which form the pore of the Ca(v2.2) channel, were similar at control, gonadectomized and gonadectomized testosterone-treated rats, suggesting that testosterone may regulate the coupling mechanisms between Ca(v2.2) and transmitter release at the neuromuscular junctions of these sexually dimorphic motoneurons.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Cacna1b  (calcium voltage-gated channel subunit alpha1 B)


Additional Information