RGD Reference Report - Atrial natriuretic peptides elevate cyclic GMP levels in primary cultures of rat ependymal cells. - Rat Genome Database

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Atrial natriuretic peptides elevate cyclic GMP levels in primary cultures of rat ependymal cells.

Authors: Wellard, J  Rapp, M  Hamprecht, B  Verleysdonk, S 
Citation: Wellard J, etal., Neurochem Res. 2003 Feb;28(2):225-33.
RGD ID: 1626242
Pubmed: PMID:12608696   (View Abstract at PubMed)

The aim of this study was to examine the effect of atrial natriuretic peptides on primary cultures of ependymal cells, as measured by changes in intracellular levels of cyclic GMP. Incubation of ependymal cells with rat atrial natriuretic peptide-(1-28) (rANP) elicited a 30-fold increase in ependymal cGMP content within 1 min and more than a 100-fold increase within 10 min to a plateau value of approximately 30 pmol/mg protein. The C-type natriuretic peptide (CNP) elicited a similar increase in cGMP levels; however the maximal effect was observed within 1 min and the levels subsequently dropped by 90% to a low plateau within 10 min. A comparison of the concentration-response curves for rANP, human ANP-(1-28) (hANP) and CNP showed that rANP, hANP and CNP had similar effects, with regards to elevation of cGMP levels at high concentrations, but with differing EC50 values. These results demonstrate the presence of a heterogenous population of functional ANP receptors i n cultured ependymalcells suggesting that ANP may regulate specific ependymal cell activity.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NppaRatpositive regulation of cGMP-mediated signaling  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NppaRathormone activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Nppa  (natriuretic peptide A)

Genes (Mus musculus)
Nppa  (natriuretic peptide type A)

Genes (Homo sapiens)
NPPA  (natriuretic peptide A)


Additional Information