RGD Reference Report - Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus. - Rat Genome Database

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Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus.

Authors: Pereira, DB  Rebola, N  Rodrigues, RJ  Cunha, RA  Carvalho, AP  Duarte, CB 
Citation: Pereira DB, etal., J Neurosci Res. 2006 Apr;83(5):832-44.
RGD ID: 1626131
Pubmed: (View Article at PubMed) PMID:16477614
DOI: Full-text: DOI:10.1002/jnr.20784

Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K(+)-evoked release of [(3)H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [(3)H]glutamate release correlated with a modest increase in phospholipase Cgamma (PLCgamma) phosphorylation, and with the lack of effect of BDNF on extracellular-signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one-third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one-fourth of total full-length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density. These results indicate that BDNF modulates synaptic transmission presynaptically in a small subset of hippocampal glutamatergic synapses that contain TrkB and that express the receptor on the plasma membrane.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Ntrk2  (neurotrophic receptor tyrosine kinase 2)


Additional Information