RGD Reference Report - Hyperglycemia- and hyperinsulinemia-induced alteration of adiponectin receptor expression and adiponectin effects in L6 myoblasts. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Hyperglycemia- and hyperinsulinemia-induced alteration of adiponectin receptor expression and adiponectin effects in L6 myoblasts.

Authors: Fang, X  Palanivel, R  Zhou, X  Liu, Y  Xu, A  Wang, Y  Sweeney, G 
Citation: Fang X, etal., J Mol Endocrinol. 2005 Dec;35(3):465-76.
RGD ID: 1625765
Pubmed: PMID:16326833   (View Abstract at PubMed)
DOI: DOI:10.1677/jme.1.01877   (Journal Full-text)

Adiponectin has been shown to regulate glucose and fatty acid uptake and metabolism in skeletal muscle. Here we investigated the role of the recently cloned adiponectin receptor (AdipoR) isoforms in mediating effects of both globular (gAd) and full-length (fAd) adiponectin, and their regulation by hyperglycemia (25 mM, 20 h) and hyperinsulinemia (100 nM, 20 h). We used L6 rat skeletal muscle cells, which were found to express both AdipoR1 and AdipoR2 mRNA in a ratio of over 6:1 respectively. Hyperglycemia and hyperinsulinemia both decreased AdipoR1 receptor expression by approximately 50%, while the latter induced an increase of approximately threefold in AdipoR2 expression. The ability of gAd to increase GLUT4 myc translocation, glucose uptake, fatty acid uptake and oxidation, as well as AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation, was decreased by both hyperglycemia and hyperinsulinemia. Interestingly, hyperinsulinemia induced the ability of fAd to elicit fatty acid uptake and enhanced fatty acid oxidation in response to fAd. In summary, our results suggest that both hyperglycemia and hyperinsulinemia cause gAd resistance in rat skeletal muscle cells. However, hyperinsulinemia induces a switch toward increased fAd sensitivity in these cells.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hyperglycemia  ISOAdipor1 (Rattus norvegicus)1625765; 1625765mRNA:decreased expression:skeletal muscle cellRGD 
hyperglycemia  IEP 1625765mRNA:decreased expression:skeletal muscle cellRGD 
hyperinsulinism  ISOAdipor1 (Rattus norvegicus)1625765; 1625765mRNA:decreased expression:skeletal muscle cellRGD 
hyperinsulinism  ISOAdipor2 (Rattus norvegicus)1625765; 1625765mRNA:decreased expression:skeletal muscle cellRGD 
hyperinsulinism  IEP 1625765mRNA:decreased expression:skeletal muscle cellRGD 
hyperinsulinism  IEP 1625765mRNA:increased expression:skeletal muscle cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Adipor1  (adiponectin receptor 1)
Adipor2  (adiponectin receptor 2)

Genes (Mus musculus)
Adipor1  (adiponectin receptor 1)
Adipor2  (adiponectin receptor 2)

Genes (Homo sapiens)
ADIPOR1  (adiponectin receptor 1)
ADIPOR2  (adiponectin receptor 2)


Additional Information