RGD Reference Report - C/EBP alpha and C/EBP beta regulate haptoglobin gene expression during rat liver development and the acute-phase response. - Rat Genome Database

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C/EBP alpha and C/EBP beta regulate haptoglobin gene expression during rat liver development and the acute-phase response.

Authors: Dinic, S  Bogojevic, D  Petrovic, M  Poznanovic, G  Ivanovic-Matic, S  Mihailovic, M 
Citation: Dinic S, etal., Mol Biol Rep. 2005 Sep;32(3):141-7.
RGD ID: 1625368
Pubmed: PMID:16172914   (View Abstract at PubMed)
DOI: DOI:10.1007/s11033-005-0750-0   (Journal Full-text)

The participation of C/EBP alpha and C/EBP beta in the transcriptional regulation of the haptoglobin (Hp) gene throughout liver development and the acute-phase (AP) response was examined. Western immunoblot analysis revealed that the relative concentrations of C/EBP alpha and C/EBP beta increased during differentiation in two nuclear protein fractions - the nuclear extract and nuclear matrix. The AP reaction was accompanied by a decrease of the relative concentration of C/EBP alpha and an increase of C/EBP beta during development in both protein fractions. Using Western analysis after DNA-affinity chromatography it was observed that a 45 kDa C/EBP alpha isoform displayed a binding affinity towards the Hp gene hormone responsive element (HRE) in both pre- and postnatal livers. In the course of the AP response DNA binding of the 45 kDa isoform was detected only in the adult, when its binding affinity decreased. The 35 kDa C/EBP beta isoform exhibited a binding affinity towards the Hp HRE after the second week from birth, whereas the AP response promoted an enhanced binding of 35 kDa isoform after the first postnatal week. These results indicate that Hp gene transcription is regulated by C/EBP alpha during normal liver development, whereas C/EBP beta is involved in the AP regulation during the later phase of differentiation and in the adult.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Acute-Phase Reaction  ISOCebpa (Rattus norvegicus)1625368; 1625368 RGD 
Acute-Phase Reaction  IEP 1625368 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acute-phase response  IEP 1625368 RGD 
liver development  IEP 1625368 RGD 
liver development  IDA 1625368 RGD 
negative regulation of DNA-templated transcription  IDA 1625368 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
nuclear matrix  IDA 1625368 RGD 
Rb-E2F complex  IDA 1625368 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex binding  IDA 1625368 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cebpa  (CCAAT/enhancer binding protein alpha)
Hp  (haptoglobin)

Genes (Mus musculus)
Cebpa  (CCAAT/enhancer binding protein alpha)

Genes (Homo sapiens)
CEBPA  (CCAAT enhancer binding protein alpha)


Additional Information