RGD Reference Report - A PLCdelta1-binding protein, p122RhoGAP, is localized in focal adhesions. - Rat Genome Database

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A PLCdelta1-binding protein, p122RhoGAP, is localized in focal adhesions.

Authors: Kawai, K  Yamaga, M  Iwamae, Y  Kiyota, M  Kamata, H  Hirata, H  Homma, Y  Yagisawa, H 
Citation: Kawai K, etal., Biochem Soc Trans. 2004 Dec;32(Pt 6):1107-9.
RGD ID: 1624304
Pubmed: PMID:15506980   (View Abstract at PubMed)
DOI: DOI:10.1042/BST0321107   (Journal Full-text)

We have investigated the cellular distribution of p122RhoGAP, a GTPase-activating protein of Rho small GTPase and an activator of phospholipase C-delta(1). Immunofluorescence studies demonstrated that endogenous p122 is localized at the tips of actin stress fibres and co-localizes with vinculin in normal rat kidney cells. In immunoprecipitation studies, p122 co-precipitated with vinculin, indicating that p122 is localized at the sites of focal adhesion. We have also shown that the N-terminal half of p122 is responsible for this localization. It is conceivable, therefore, that p122 is involved in the reorganization of the actin cytoskeleton and focal adhesions that regulate cell-substratum adhesion and cell migration.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin filament located_inIDA 1624304; 1624304 RGD 
focal adhesion located_inIDA 1624304; 1624304 RGD 
stress fiber located_inIDA 1624304; 1624304 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
small GTPase binding  IPIDlc1 (Rattus norvegicus)1624304 RGD 
vinculin binding  IPIVcl (Rattus norvegicus)1624304 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dlc1  (DLC1 Rho GTPase activating protein)
Vcl  (vinculin)


Additional Information