RGD Reference Report - A region-specific increase in Galphaq and Galpha11 proteins in brains of rats during cocaine withdrawal. - Rat Genome Database

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A region-specific increase in Galphaq and Galpha11 proteins in brains of rats during cocaine withdrawal.

Authors: Carrasco, GA  Zhang, Y  Damjanoska, KJ  D'Souza, DN  Garcia, F  Battaglia, G  Muma, NA  Van de Kar, LD 
Citation: Carrasco GA, etal., J Pharmacol Exp Ther. 2003 Dec;307(3):1012-9. Epub 2003 Oct 8.
RGD ID: 1601365
Pubmed: (View Article at PubMed) PMID:14534355
DOI: Full-text: DOI:10.1124/jpet.103.056978

Serotonin 2A (5-HT2A) receptor-mediated increases in plasma hormone levels become supersensitive after 42 h of withdrawal from cocaine treatment. The present study investigated which components of the 5-HT2A receptor signaling system are associated with this supersensitivity. Rats were injected daily for 14 days with either saline or cocaine (15 mg/kg i.p.) twice a day or were injected using a "binge" protocol (three injections per day, 1 h apart). Rats were sacrificed 2 or 7 days after the last cocaine injection, and the levels of membrane and cytosol-associated 5-HT2A receptors, Galphaq, Galpha11, regulators of G protein signaling (RGS)4, and RGS7 proteins were assayed in the hypothalamic paraventricular nucleus, amygdala, and frontal cortex using Western blot analysis. Two days of withdrawal from cocaine, administered twice a day or using a binge protocol, produced an increase in membrane-associated Galphaq and Galpha11 proteins in the paraventricular nucleus and the amygdala (but not in the frontal cortex). This effect was reversible after 7 days of withdrawal. The protein levels of the 5-HT2A receptor, Galphaz protein, and RGS4 or RGS7 proteins were not altered by cocaine withdrawal in any of the above-mentioned brain regions. These findings suggest that the supersensitivity of the 5-HT2A receptors, during withdrawal from chronic cocaine, is associated with an increase in membrane-associated Galphaq and Galpha11 proteins and not with changes in the expression of 5-HT2A receptors.



Gene Ontology Annotations    

Cellular Component
cytosol  (IDA)
plasma membrane  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Gna11  (G protein subunit alpha 11)
Gnaq  (G protein subunit alpha q)
Gnaz  (G protein subunit alpha z)
Htr2a  (5-hydroxytryptamine receptor 2A)
Rgs4  (regulator of G-protein signaling 4)
Rgs7  (regulator of G-protein signaling 7)


Additional Information