RGD Reference Report - Mechanisms of orexin A-evoked changes of intracellular calcium in primary cultured cortical neurons. - Rat Genome Database

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Mechanisms of orexin A-evoked changes of intracellular calcium in primary cultured cortical neurons.

Authors: Xia, JX  Chen, XW  Cheng, SY  Hu, ZA 
Citation: Xia JX, etal., Neuroreport. 2005 May 12;16(7):783-6.
RGD ID: 1600997
Pubmed: (View Article at PubMed) PMID:15858425

We have investigated the effect of orexin A on the intracellular free calcium concentration ([Ca2+]i) in primary cultured cortical neurons and explored the exact mechanisms of orexin A-evoked changes of [Ca2+]i. In the present study, changes of [Ca2+]i induced by orexin A in primary cultured cortical neurons were first detected by confocal laser scanning microscopy using Ca2+-sensitive dye fluo-4 as a novel calcium fluorescent probe. Our results showed that 1-0.1 microM orexin A induced the increase in [Ca2+]i in cortical neurons. The increase in [Ca2+]i by acute application of orexin A occurred in a dose-dependent manner. Orexin A-induced increase in [Ca2+]i was not observed under the condition of Ca2+-free Dulbecco's modified Eagle's medium. Pretreatment on the cells with 1 microM thapsigargin did not block orexin A-evoked response. These findings first illuminated the fact that orexin A-induced increase in [Ca2+]i may be mainly from extracellular calcium influx in cortical neurons.

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Genes (Rattus norvegicus)
Hcrt  (hypocretin neuropeptide precursor)


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