RGD Reference Report - Overexpression of lysyl oxidase to increase matrix crosslinking and improve tissue strength in dermal wound healing. - Rat Genome Database

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Overexpression of lysyl oxidase to increase matrix crosslinking and improve tissue strength in dermal wound healing.

Authors: Lau, YK  Gobin, AM  West, JL 
Citation: Lau YK, etal., Ann Biomed Eng. 2006 Aug;34(8):1239-46. Epub 2006 Jun 28.
RGD ID: 1600668
Pubmed: PMID:16804742   (View Abstract at PubMed)
DOI: DOI:10.1007/s10439-006-9130-8   (Journal Full-text)

In this study, we aimed to increase crosslinking in collagen and elastin in the extracellular matrix through overexpression of lysyl oxidase (LO) in order to improve mechanical strength in dermal wounds during healing. We had used a gene activated matrix (GAM) approach to locally deliver plasmid DNA (pDNA) complexed with polyethylenimine (PEI) in collagen gels at the wound site for localized and sustained transfection of cells involved in the healing process. We first demonstrated in vitro that PEI-pDNA complexes in collagen gels could be taken up and expressed by cultured fibroblasts for at least 20 days. In vitro studies showed that fibroblast-seeded GAMs with the LO transgene exhibited over a 3-fold increase in mechanical strength as compared with a green fluorescent protein (GFP)-transgene control. Addition of an inhibitor of LO abolished this increase. We applied this system in a rat dermal wound healing model and showed that treatment with LO-producing GAMs led to significantly enhanced mechanical strength of the wound site.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LoxRatcollagen fibril organization  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LoxRatprotein-lysine 6-oxidase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lox  (lysyl oxidase)


Additional Information