RGD Reference Report - Identification of a multiprotein "motor" complex binding to water channel aquaporin-2. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Identification of a multiprotein "motor" complex binding to water channel aquaporin-2.

Authors: Noda, Y  Horikawa, S  Katayama, Y  Sasaki, S 
Citation: Noda Y, etal., Biochem Biophys Res Commun. 2005 May 20;330(4):1041-7.
RGD ID: 1600561
Pubmed: PMID:15823548   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2005.03.079   (Journal Full-text)

Targeted positioning of water channel aquaporin-2 (AQP2) strictly regulates body water homeostasis. Trafficking of AQP2 to the apical membrane is critical to the reabsorption of water in renal collecting ducts. Recently, we have identified for the first time proteins which directly bind to AQP2: SPA-1, a GTPase-activating protein for Rap1, and cytoskeletal protein actin. Based on these findings, we have speculated the existence of a multiprotein complex which includes AQP2, SPA-1, and actin, for providing the mechanism which generates force and motion in AQP2 trafficking. To clarify the proteins comprising the complex, a large amount of AQP2-associated protein complex was isolated from the extract of rat kidney papilla using immunoaffinity column coupled with anti-AQP2 antibody and was analyzed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition to SPA-1 and actin, 11 proteins were identified using this method: ionized calcium binding adapter molecule 2, myosin regulatory light chain smooth muscle isoforms 2-A and 2-B, alpha-tropomyosin 5b, annexin A2 and A6, scinderin, gelsolin, alpha-actinin 4, alpha-II spectrin, and myosin heavy chain nonmuscle type A. Our findings show for the first time an AQP2-binding multiprotein "force generator" complex. This multiprotein complex may provide the machinery of driving AQP2 movement.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex  IDA 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561; 1600561 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Actb  (actin, beta)
Actn4  (actinin alpha 4)
Aif1l  (allograft inflammatory factor 1-like)
Anxa2  (annexin A2)
Anxa6  (annexin A6)
Aqp2  (aquaporin 2)
Gsn  (gelsolin)
Myh9  (myosin, heavy chain 9)
Myl12a  (myosin light chain 12A)
Myl12b  (myosin light chain 12B)
Scin  (scinderin)
Sipa1l1  (signal-induced proliferation-associated 1 like 1)
Sptan1  (spectrin, alpha, non-erythrocytic 1)
Tpm1  (tropomyosin 1)
Tpm1_v8  (tropomyosin 1, variant 8)

Objects referenced in this article
Gene Tpm1_v1 tropomyosin 1, variant 1 Rattus norvegicus
Gene Tpm1_v2 tropomyosin 1, variant 2 Rattus norvegicus
Gene Tpm1_v3 tropomyosin 1, variant 3 Rattus norvegicus
Gene Tpm1_v4 tropomyosin 1, variant 4 Rattus norvegicus
Gene Tpm1_v5 tropomyosin 1, variant 5 Rattus norvegicus
Gene Tpm1_v6 tropomyosin 1, variant 6 Rattus norvegicus
Gene Tpm1_v7 tropomyosin 1, variant 7 Rattus norvegicus
Gene Tpm1_v9 tropomyosin 1, variant 9 Rattus norvegicus

Additional Information