RGD Reference Report - Voltage-dependent Calcium Channel Abnormalities in Hippocampal CA3 Neurons of Spontaneously Epileptic Rats. - Rat Genome Database

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Voltage-dependent Calcium Channel Abnormalities in Hippocampal CA3 Neurons of Spontaneously Epileptic Rats.

Authors: Yan, HD  Ishihara, K  Hanaya, R  Kurisu, K  Serikawa, T  Sasa, M 
Citation: Yan HD, etal., Epilepsia. 2007 Feb 22;.
RGD ID: 1600256
Pubmed: PMID:17326796   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1528-1167.2007.00957.x   (Journal Full-text)

Purpose: Hippocampal CA3 neurons of spontaneously epileptic rats (SER; zi/zi, tm/tm), which show both absence-like seizures and tonic convulsions, exhibit a long-lasting depolarization shift with repetitive firing with a single stimulation of mossy fibers. Therefore a whole-cell patch-clamp study using temporarily dissociated hippocampal CA3 neurons from SER was performed to elucidate whether such abnormal excitability was due to abnormalities in voltage-dependent Ca(2+) channels (VDCCs). Methods: Hippocampal CA3 neurons were temporarily dissociated with enzymatic and mechanical treatments. In a voltage-clamp mode with whole-cell recording, depolarizing step pulses were applied to induce Ca(2+) currents in the presence of tetrodotoxin and tetraethylammonium. Results: The threshold level of the Ca(2+) current induced by depolarizing pulses was found to be lower in hippocampal CA3 neurons of SER compared with those of control Wistar rats. In addition, the Ca(2+) current peak amplitude was greater, and decay of the current was weaker in CA3 neurons of SER than in those of normal Wistar rats. Conclusions: These findings suggest that enhancements of Ca(2+) influx into hippocampal CA3 neurons due to the easier activation properties of VDCCs, as well as a decrease in decay, are involved in SER epileptic seizures.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
visual epilepsy MODEL:spontaneousIAGP 1600256compared to Wistar ratsRGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
abnormal calcium ion homeostasis  IAGP 1600256compared to Wistar ratsRGD 
abnormal summary potential threshold  IAGP 1600256compared to Wistar ratsRGD 
absence seizures  IAGP 1600256compared to Wistar ratsRGD 
convulsive seizures  IAGP 1600256compared to Wistar ratsRGD 
increased excitatory postsynaptic current amplitude  IAGP 1600256compared to Wistar ratsRGD 
short excitatory postsynaptic current decay time  IAGP 1600256compared to Wistar ratsRGD 
tonic seizures  IAGP 1600256compared to Wistar ratsRGD 
Objects Annotated

Strains
SER/Kyo  (spontaneously epileptic rat)


Additional Information