RGD Reference Report - Increased expression of the sodium transporter BSC-1 in spontaneously hypertensive rats. - Rat Genome Database

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Increased expression of the sodium transporter BSC-1 in spontaneously hypertensive rats.

Authors: Sonalker, PA  Tofovic, SP  Jackson, EK 
Citation: Sonalker PA, etal., J Pharmacol Exp Ther. 2004 Dec;311(3):1052-61. Epub 2004 Aug 30.
RGD ID: 1600028
Pubmed: PMID:15340004   (View Abstract at PubMed)
DOI: DOI:10.1124/jpet.104.071209   (Journal Full-text)

The purpose of this study was to compare the expression of BSC-1 (bumetanide-sensitive Na+-K+-2Cl- cotransporter) in kidneys of spontaneously hypertensive rats (SHR) versus Wistar-Kyoto (WKY) rats by immunoblotting and reverse transcription-polymerase chain reaction. To determine the specificity of any observed changes in BSC-1 expression, we also compared expression of the thiazide sensitive Na+-Cl- cotransporter (TSC), the type-3 Na+-H+ exchanger (NHE-3), Na+-K+-ATPase-alpha1, the inwardly rectifying K+ channel (ROMK-1), the type-1 Na+-HCO3- cotransporter (NBC-1), aquaporin-1, and aquaporin-2. Analyses were performed on outer cortex, outer medulla, and inner medulla. BSC-1 protein was detected in outer medulla and was markedly (6-fold) higher in SHR. TSC protein was detected in the cortex and was not overexpressed in SHR. Aquaporin-1 protein was detected in all three regions and was not overexpressed in SHR. Aquaporin-2 and ROMK-1 proteins were detected in all three regions, but were moderately elevated (2-fold) only in the SHR inner medulla. Na+-K+-ATPase and NHE-3 proteins were detected in all three regions. Na+-K+-ATPase-alpha1 was modestly (25%) increased in SHR outer and inner medulla, whereas NHE-3 was moderately (2-fold) increased in the SHR cortex and inner medulla. NBC-1 protein was detected only in the cortex and was higher (2-fold) in SHR. mRNA levels of BSC-1, aquaporin-2, and ROMK-1 were not elevated in SHR, indicating a post-translational mechanism of protein overexpression. High-dose furosemide increased fractional sodium excretion more in SHR than WKY (3-fold). We conclude that increased expression of BSC-1, and to a lesser extent, aquaporin-2, ROMK-1, NHE-3, and NBC-1 may contribute to the pathogenesis of hypertension in the SHR.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypertension  ISOSlc4a4 (Rattus norvegicus)1600028; 1600028protein:increased expression:kidney cortexRGD 
hypertension  IEP 1600028protein:increased expression:kidney cortexRGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc4a4  (solute carrier family 4 member 4)

Genes (Mus musculus)
Slc4a4  (solute carrier family 4 (anion exchanger), member 4)

Genes (Homo sapiens)
SLC4A4  (solute carrier family 4 member 4)


Additional Information