RGD Reference Report - Botulinum-induced muscle paralysis alters metabolic gene expression and fatigue recovery. - Rat Genome Database

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Botulinum-induced muscle paralysis alters metabolic gene expression and fatigue recovery.

Authors: Gorin, F  Herrick, K  Froman, B  Palmer, W  Tait, R  Carlsen, R 
Citation: Gorin F, etal., Am J Physiol. 1996 Jan;270(1 Pt 2):R238-45.
RGD ID: 1599993
Pubmed: PMID:8769807   (View Abstract at PubMed)

We evaluated the physiological, histochemical, and biochemical consequences of inhibiting contractile activity in rat skeletal muscles with botulinum toxin A (BTX). Contractile activity was entirely eliminated 12-18 h after a single, focal, intramuscular injection of BTX into the rat tibialis anterior muscle (TA). Neuromuscular transmission remained completely inhibited for 10-12 days, then slowly recovered. BTX-treated muscles exhibited a lower resistance to both high- and low-frequency fatigue at 7 and 14 days after injection, but contractile force recovered more rapidly in treated TA after fatigue. Treated TA showed a twofold increase in the activity of the triglyceride hydrolase enzyme lipoprotein lipase (LPL) and a comparable increase in the relative abundance of LPL steady-state mRNA. In contrast, there was a 28% reduction in protein levels of the muscle isozyme of glycogen phosphorylase (MGP) and a 70% decrease in relative MGP transcript levels. Similar changes in relative transcript levels of LPL and MGP were observed in the predominantly fast-twitch extensor digitorum longus after BTX injection, but relative LPL and MGP mRNA levels were not altered in predominantly slow-twitch soleus. Histochemical evidence indicated that fast-twitch glycolytic fibers had increased lipid content. These biochemical alterations were reversed 120 days after BTX treatment despite persistent atrophy.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PYGMHumanParalysis  ISOPygm (Rattus norvegicus)mRNA and protein:decreased expression:skeletal muscleRGD 
PygmRatParalysis  IEP mRNA and protein:decreased expression:skeletal muscleRGD 
PygmMouseParalysis  ISOPygm (Rattus norvegicus)mRNA and protein:decreased expression:skeletal muscleRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pygm  (glycogen phosphorylase, muscle associated)

Genes (Mus musculus)
Pygm  (muscle glycogen phosphorylase)

Genes (Homo sapiens)
PYGM  (glycogen phosphorylase, muscle associated)


Additional Information