RGD Reference Report - Gene expression profile of cardiomyocytes in hypertrophic heart induced by continuous norepinephrine infusion in the rats. - Rat Genome Database

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Gene expression profile of cardiomyocytes in hypertrophic heart induced by continuous norepinephrine infusion in the rats.

Authors: Li, P  Li, J  Feng, X  Li, Z  Hou, R  Han, C  Zhang, Y 
Citation: Li P, etal., Cell Mol Life Sci. 2003 Oct;60(10):2200-9.
RGD ID: 1599987
Pubmed: PMID:14618266   (View Abstract at PubMed)
DOI: DOI:10.1007/s00018-003-3178-5   (Journal Full-text)

Catecholamines play an important role in the development of cardiac hypertrophy. To observe cardiomyocyte-specific gene expression changes induced by catecholamines in vivo, left ventricular cardiomyocytes were isolated from male Sprague-Dawley rats after continuous infusion of norepinephrine (NE; 0.2 mg/kg per hour intravenously) for 0.5, 1, 2, 3 and 7 days. The gene expression profiles of these cells during different NE infusion stages were assessed by using a cDNA microarray, and the microarray data were further analyzed by a clustering method. Cardiac hypertrophy was induced upon continuous NE infusion, with the peak at 3 days. Meanwhile, manifest changes in gene expression profile within cardiomyocytes over the time course were revealed, most of the genes never having been reported to be involved in cardiac hypertrophy. The number of genes displaying differential expression also peaked at the middle stage of infusion (2-3 days), and the majority of the signaling molecules were found differentially expressed mainly at this stage, including phosphatidylinositol 3-kinase, calcium/calmodulin-dependent protein kinase II and non-receptor tyrosine kinases, etc. The tumor suppressor p53 was found up-regulated at very early (0.5 days) and late stages (7 days) of NE infusion. Self-organization clustering analysis revealed subsets of coordinate regulated genes. One set consisted of several enzymes involved in energy metabolism, including carnitine octanoyltransferase, ATP synthase subunit c, pancreatic lipase and glycogen phosphorylase, possessing a similar expression pattern with a rapidly elevated expression level at the early stage of NE infusion. This is the first study to provide transcriptional information for cardiomyocytes, a single cell type, in the heart during the development of cardiac hypertrophy in vivo, and may provide accurate clues to elaborate hypotheses about the evolution of this pathology.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CROTHumanLeft Ventricular Hypertrophy  ISOCrot (Rattus norvegicus)mRNA:increased expression:heart left ventricle and cardiac muscle cellRGD 
CrotRatLeft Ventricular Hypertrophy  HEP mRNA:increased expression:heart left ventricle and cardiac muscle cellRGD 
CrotMouseLeft Ventricular Hypertrophy  ISOCrot (Rattus norvegicus)mRNA:increased expression:heart left ventricle and cardiac muscle cellRGD 
PYGMHumanLeft Ventricular Hypertrophy  ISOPygm (Rattus norvegicus)mRNA:increased expression:heart left ventricle and cardiac muscle cellRGD 
PygmRatLeft Ventricular Hypertrophy  HEP mRNA:increased expression:heart left ventricle and cardiac muscle cellRGD 
PygmMouseLeft Ventricular Hypertrophy  ISOPygm (Rattus norvegicus)mRNA:increased expression:heart left ventricle and cardiac muscle cellRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CrotRatcellular response to norepinephrine stimulus  HEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crot  (carnitine O-octanoyltransferase)
Pygm  (glycogen phosphorylase, muscle associated)

Genes (Mus musculus)
Crot  (carnitine O-octanoyltransferase)
Pygm  (muscle glycogen phosphorylase)

Genes (Homo sapiens)
CROT  (carnitine O-octanoyltransferase)
PYGM  (glycogen phosphorylase, muscle associated)

Objects referenced in this article
Gene COX8BP cytochrome c oxidase subunit 8B, pseudogene Homo sapiens
Gene Cox8b cytochrome c oxidase subunit 8B Mus musculus
Gene Cox8b cytochrome c oxidase, subunit VIIIb Rattus norvegicus

Additional Information