RGD Reference Report - The content of glycogen phosphorylase and glycogen in preparations of sarcoplasmic reticulum-glycogenolytic complex is enhanced in diabetic rat skeletal muscle. - Rat Genome Database

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The content of glycogen phosphorylase and glycogen in preparations of sarcoplasmic reticulum-glycogenolytic complex is enhanced in diabetic rat skeletal muscle.

Authors: Garduno, E  Nogues, M  Merino, JM  Gutierrez-Merino, C  Henao, F 
Citation: Garduno E, etal., Diabetologia. 2001 Oct;44(10):1238-46.
RGD ID: 1599897
Pubmed: PMID:11692172   (View Abstract at PubMed)
DOI: DOI:10.1007/s001250100637   (Journal Full-text)

AIMS/HYPOTHESIS: We have examined the effect of diabetes and pharmacological insulin treatment on the content of glycogen phosphorylase and glycogen associated with the sarcoplasmic reticulum-glycogenolytic complex from rat skeletal muscle. METHODS: Diabetes was induced in rats by streptozotocin injection. Enzymatic activities were measured using spectrophotometric methods. Glycogen phosphorylase was determined measuring the pyridoxal-5' -phosphate content and using polyacrylamide gel electrophoresis. Glycogen content was measured by enzymatic and the phenol sulfuric methods. RESULTS: The content of glycogen phosphorylase associated with the sarcoplasmic reticulum glycogenolytic complex gradually arises after diabetes induction. The content of glycogen phosphorylase was restored to a control value by pharmacological insulin treatment. In addition, the content of glycogen in preparations of sarcoplasmic reticulum-glycogenolytic complex of diabetic animals was also increased, whereas the content of glycogen in total muscle of diabetic rats was similar to that of the control rats. The absolute and relative amount of glycogen associated with sarcoplasmic reticulum seemed to increase in diabetic animals. These effects on the compartmentalisation of glycogen were suppressed by insulin treatment. Additionally, the rate of conversion of glycogen phosphorylase b to a, an index of the phosphorylase kinase activity, was 50 % lower in diabetic rats, increasing the dephosphorylated form of glycogen phosphorylase and, as a consequence, its association with sarcoplasmic reticulum membranes. CONCLUSION/INTERPRETATION: These results suggest that under diabetic conditions, both glycogen phosphorylase and a small percentage of muscle glycogen are relocalized in the sarcoplasmic reticulum-glycogenolytic complex.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PHKA1HumanExperimental Diabetes Mellitus  ISOPhka1 (Rattus norvegicus) RGD 
PYGMHumanExperimental Diabetes Mellitus  ISOPygm (Rattus norvegicus)protein:increased expression:sarcoplasmic reticulumRGD 
Phka1RatExperimental Diabetes Mellitus  IDA  RGD 
Phka1MouseExperimental Diabetes Mellitus  ISOPhka1 (Rattus norvegicus) RGD 
PygmRatExperimental Diabetes Mellitus  IDA protein:increased expression:sarcoplasmic reticulumRGD 
PygmMouseExperimental Diabetes Mellitus  ISOPygm (Rattus norvegicus)protein:increased expression:sarcoplasmic reticulumRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PygmRatglycogen catabolic process  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PygmRatsarcoplasmic reticulum  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PygmRatglycogen phosphorylase activity  IDA  RGD 
PygmRatpyridoxal phosphate binding  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PYGMHumanglycogen degradation pathway   ISOPygm (Rattus norvegicus) RGD 
PygmRatglycogen degradation pathway   IDA  RGD 
PygmMouseglycogen degradation pathway   ISOPygm (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Phka1  (phosphorylase kinase regulatory subunit alpha 1)
Pygm  (glycogen phosphorylase, muscle associated)

Genes (Mus musculus)
Phka1  (phosphorylase kinase alpha 1)
Pygm  (muscle glycogen phosphorylase)

Genes (Homo sapiens)
PHKA1  (phosphorylase kinase regulatory subunit alpha 1)
PYGM  (glycogen phosphorylase, muscle associated)


Additional Information