RGD Reference Report - Genomics of the periinfarction cortex after focal cerebral ischemia. - Rat Genome Database

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Genomics of the periinfarction cortex after focal cerebral ischemia.

Authors: Lu, A  Tang, Y  Ran, R  Clark, JF  Aronow, BJ  Sharp, FR 
Citation: Lu A, etal., J Cereb Blood Flow Metab. 2003 Jul;23(7):786-810.
RGD ID: 1599640
Pubmed: PMID:12843783   (View Abstract at PubMed)
DOI: DOI:10.1097/01.WCB.0000062340.80057.06   (Journal Full-text)

Understanding transcriptional changes in brain after ischemia may provide therapeutic targets for treating stroke and promoting recovery. To study these changes on a genomic scale, oligonucleotide arrays were used to assess RNA samples from periinfarction cortex of adult Sprague-Dawley rats 24 h after permanent middle cerebral artery occlusions. Of the 328 regulated transcripts in ischemia compared with sham-operated animals, 264 were upregulated, 64 were downregulated, and 163 (49.7%) had not been reported in stroke. Of the functional groups modulated by ischemia: G-protein-related genes were the least reported; and cytokines, chemokines, stress proteins, and cell adhesion and immune molecules were the most highly expressed. Quantitative reverse transcription polymerase chain reaction of 20 selected genes at 2, 4, and 24 h after ischemia showed early upregulated genes (2 h) including Narp, Rad, G33A, HYCP2, Pim-3, Cpg21, JAK2, CELF, Tenascin, and DAF. Late upregulated genes (24 h) included Cathepsin C, Cip-26, Cystatin B, PHAS-I, TBFII, Spr, PRG1, and LPS-binding protein. Glycerol 3-phosphate dehydrogenase, which is involved in mitochondrial reoxidation of glycolysis derived NADH, was regulated more than 60-fold. Plasticity-related transcripts were regulated, including Narp, agrin, and Cpg21. A newly reported lung pathway was also regulated in ischemic brain: C/EBP induction of Egr-1 (NGFI-A) with downstream induction of PAI-1, VEGF, ICAM, IL1, and MIP1. Genes regulated acutely after stroke may modulate cell survival and death; also, late regulated genes may be related to tissue repair and functional recovery.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
brain ischemia  ISOCtsc (Rattus norvegicus)1599640; 1599640mRNA:increased expression:cerebral cortexRGD 
brain ischemia  IEP 1599640mRNA:increased expression:cerebral cortexRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ctsc  (cathepsin C)

Genes (Mus musculus)
Ctsc  (cathepsin C)

Genes (Homo sapiens)
CTSC  (cathepsin C)


Additional Information