RGD Reference Report - Capillary electrophoresis assay of alanine:glyoxylate aminotransferase activity in rat liver. - Rat Genome Database

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Capillary electrophoresis assay of alanine:glyoxylate aminotransferase activity in rat liver.

Authors: Nishijima, S  Sugaya, K  Morozumi, M  Hatano, T  Ogawa, Y 
Citation: Nishijima S, etal., J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Nov 15;780(1):13-9.
RGD ID: 1599457
Pubmed: PMID:12383475   (View Abstract at PubMed)

We measured hepatic alanine:glyoxylate aminotransferase (AGT) activity using capillary electrophoresis. After rat liver homogenate was incubated in the presence of substrates and pyridoxal 5'-phosphate, the pyruvate and glycine produced by AGT were measured. The AGT activity was 10.02+/-0.31 micro mol pyruvate/h/mg protein and 10.21+/-0.15 micro mol glycine/h/mg protein. This method is relatively simple and shows superior sensitivity, allowing the measurement of enzyme activity in 5 micro g of protein. Therefore, it appears to be suitable for laboratory use and may also have advantages for measuring AGT activity in liver biopsy specimens.



Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Agxt  (alanine--glyoxylate aminotransferase)


Additional Information