RGD Reference Report - Regulation of genes for inducible nitric oxide synthase and urea cycle enzymes in rat liver in endotoxin shock. - Rat Genome Database

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Regulation of genes for inducible nitric oxide synthase and urea cycle enzymes in rat liver in endotoxin shock.

Authors: Tabuchi, S  Gotoh, T  Miyanaka, K  Tomita, K  Mori, M 
Citation: Tabuchi S, etal., Biochem Biophys Res Commun. 2000 Feb 5;268(1):221-4.
RGD ID: 1599265
Pubmed: PMID:10652239   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2000.2105   (Journal Full-text)

Arginine is an intermediate of the urea cycle in the liver. It is synthesized by the first four enzymes of the cycle, carbamylphosphate synthetase I, ornithine transcarbamylase, argininosuccinate synthetase, and argininosuccinate lyase, and is hydrolyzed to urea and ornithine by arginase I, forming the cycle. In endotoxemia shock, inducible nitric oxide (NO) synthase (iNOS) is induced in hepatocytes and arginine is utilized for NO production. Regulation of the genes for iNOS and the urea cycle enzymes was studied using lipopolysaccharide (LPS)-treated rat livers. When rats were injected intraperitoneally with LPS, iNOS mRNA was markedly induced. Cationic amino acid transporter-2 and C/EBPbeta mRNAs were also highly increased. In contrast, mRNAs for all the urea cycle enzymes except ornithine transcarbamylase were gradually decreased and reached 16-28% of controls at 12 h. However, all these enzymes remained unchanged at protein level up to 24 h. In light of these results, we suggest that synthesis of urea cycle enzymes is downregulated and that the protein synthetic capacity is directed to synthesis of proteins required for defense against endotoxemia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Endotoxemia  ISOAsl (Rattus norvegicus)1599265; 1599265 RGD 
Endotoxemia  ISOAss1 (Rattus norvegicus)1599265; 1599265 RGD 
Endotoxemia  IEP 1599265; 1599265 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to lipopolysaccharide  IEP 1599265; 1599265 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arg1  (arginase 1)
Asl  (argininosuccinate lyase)
Ass1  (argininosuccinate synthase 1)

Genes (Mus musculus)
Asl  (argininosuccinate lyase)
Ass1  (argininosuccinate synthetase 1)

Genes (Homo sapiens)
ASL  (argininosuccinate lyase)
ASS1  (argininosuccinate synthase 1)


Additional Information