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The effect of pH on the kinetics of arylsulphatases A and B.

Authors: O'Fagain, C  Butler, BM  Mantle, TJ 
Citation: O'Fagain C, etal., Biochem J. 1983 Sep 1;213(3):603-7.
Pubmed: (View Article at PubMed) PMID:6137211

The effect of pH on the kinetics of rat liver arylsulphatases A and B is very similar and shows that two groups with pK values of 4.4-4.5 and 5.7-5.8 are important for enzyme activity. Substrate binding has no effect on the group with a pK of 4.4-4.5; however, the pK of the second group is shifted to 7.1-7.5 in the enzyme-substrate complex. An analysis of the effect of pH on the Ki for sulphate inhibition suggests that HSO4-is the true product. A model is proposed that involves the two ionizing groups identified in the present study in a concerted general acid-base-catalysed mechanism.


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RGD Object Information
RGD ID: 1599227
Created: 2007-01-22
Species: All species
Last Modified: 2007-01-22
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.