RGD Reference Report - Mechanism-based inhibition of proline dehydrogenase by proline analogues. - Rat Genome Database

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Mechanism-based inhibition of proline dehydrogenase by proline analogues.

Authors: Tritsch, D  Mawlawi, H  Biellmann, JF 
Citation: Tritsch D, etal., Biochim Biophys Acta. 1993 Sep 3;1202(1):77-81.
RGD ID: 1599203
Pubmed: PMID:8373828   (View Abstract at PubMed)

The inactivation of proline dehydrogenase by several L-Pro analogues was investigated with the aim to block the essential metabolic pathway of tsetse flies allowing the degradation of L-Pro to L-Glu. In vitro studies on rat liver mitochondria showed that only 4-methylene-L-proline was able to inactivate proline dehydrogenase. The inactivation kinetics agreed with a mechanism-based inhibition. The other tested analogues E- and Z-4-fluoromethylene-L-proline, and cis and trans-5-ethynyl-D,L-proline were neither substrate nor inactivator of the enzyme. In vivo 4-methylene-L-proline showed no toxicity against Drosophila flies, but was lethal for Glossina pallidipes flies. This result allows the consideration of 4-methylene-L-proline as an attractive compound molecule in the struggle against tsetse flies.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
amino acid binding  IMP 1599203 RGD 
proline dehydrogenase activity  IMP 1599203 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prodh  (proline dehydrogenase)


Additional Information