RGD Reference Report - The mitochondrial location of protoporphyrinogen oxidase. - Rat Genome Database

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The mitochondrial location of protoporphyrinogen oxidase.

Authors: Deybach, JC  Da Silva, V  Grandchamp, B  Nordmann, Y 
Citation: Deybach JC, etal., Eur J Biochem. 1985 Jun 3;149(2):431-5.
RGD ID: 1599183
Pubmed: PMID:3996415   (View Abstract at PubMed)

Using the digitonin method and subsequent fractionation of rat liver mitochondria, protoporphyrinogen oxidase (penultimate enzyme in the heme biosynthesis pathway) was found to be closely associated with the mitochondrial inner membrane fraction. Chemical treatment with non-specific probes (trypsin and diazobenzene sulfonate) of either intact or inverted mitoplasts, indicated that protoporphyrinogen oxidase was anchored within the lipid bilayer of the inner membrane. Protoporphyrinogen had an equal access to the active site of the enzyme from both sides of the inner membrane and its transformation to protoporphyrin did not appear to be energy-dependent. Studies of protoporphyrinogen synthesis from exogenously added coproporphyrinogen in either intact or hypoosmotically treated mitochondria underlined the importance of the peculiar submitochondrial location of coproporphyrinogen oxidase and protoporphyrinogen oxidase for the transfer of substrates to the inner membrane.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial inner membrane  IDA 1599183 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ppox  (protoporphyrinogen oxidase)

Objects referenced in this article
Gene Cpox coproporphyrinogen oxidase Rattus norvegicus

Additional Information