RGD Reference Report - An h.p.l.c. assay for protoporphyrinogen oxidase activity in rat liver. - Rat Genome Database

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An h.p.l.c. assay for protoporphyrinogen oxidase activity in rat liver.

Authors: Li, F  Lim, CK  Peters, TJ 
Citation: Li F, etal., Biochem J. 1987 May 1;243(3):863-6.
RGD ID: 1599180
Pubmed: PMID:3663105   (View Abstract at PubMed)
PMCID: PMC1147937   (View Article at PubMed Central)

An h.p.l.c. method is described for the assay of protoporphyrinogen oxidase activity in rat liver. A relatively pure protoporphyrinogen IX substrate was obtained by selectively removing any protoporphyrin IX unreduced by sodium amalgam on a small disposable cartridge packed with a strong anion-exchanger. The protoporphyrin IX formed was extracted with dimethyl sulphoxide/methanol (3:7, v/v) containing mesoporphyrin as the internal standard for separation and quantification by reversed-phase chromatography. The Km for protoporphyrinogen was 9.5 +/- 1.6 microM, and the enzyme activities were 0.59 +/- 0.11 nmol of protoporphyrin IX produced/min per mg of mitochondrial protein and 33.5 +/- 2.7 nmol protoporphyrin IX produced/min per g of liver tissue homogenate. The method is applicable to the determination of enzyme activity in small amounts of human liver biopsy.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
porphyrin-containing compound biosynthetic process  IDA 1599180 RGD 
protoporphyrinogen IX metabolic process  IDA 1599180 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
oxygen-dependent protoporphyrinogen oxidase activity  IDA 1599180 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
heme biosynthetic pathway   ISOPpox (Rattus norvegicus)1599180; 1599180 RGD 
heme biosynthetic pathway   IDA 1599180 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Ppox  (protoporphyrinogen oxidase)

Genes (Mus musculus)
Ppox  (protoporphyrinogen oxidase)

Genes (Homo sapiens)
PPOX  (protoporphyrinogen oxidase)

Additional Information