RGD Reference Report - t(3;11) translocation in treatment-related acute myeloid leukemia fuses MLL with the GMPS (GUANOSINE 5' MONOPHOSPHATE SYNTHETASE) gene. - Rat Genome Database

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t(3;11) translocation in treatment-related acute myeloid leukemia fuses MLL with the GMPS (GUANOSINE 5' MONOPHOSPHATE SYNTHETASE) gene.

Authors: Pegram, LD  Megonigal, MD  Lange, BJ  Nowell, PC  Rowley, JD  Rappaport, EF  Felix, CA 
Citation: Pegram LD, etal., Blood. 2000 Dec 15;96(13):4360-2.
RGD ID: 1598998
Pubmed: (View Article at PubMed) PMID:11110714

The partner gene of MLL was identified in a patient with treatment-related acute myeloid leukemia in which the karyotype suggested t(3;11)(q25;q23). Prior therapy included the DNA topoisomerase II inhibitors, teniposide and doxorubicin. Southern blot analysis indicated that the MLL gene was involved in the translocation. cDNA panhandle polymerase chain reaction (PCR) was used, which does not require partner gene-specific primers, to identify the chimeric transcript. Reverse-transcription of first-strand cDNAs with oligonucleotides containing known MLL sequence at the 5' ends and random hexamers at the 3' ends generated templates with an intra-strand loop for PCR. In-frame fusions of either MLL exon 7 or exon 8 with the GMPS (GUANOSINE 5'-MONOPHOSPHATE SYNTHETASE) gene from chromosome band 3q24 were detected. The fusion transcript was alternatively spliced. Guanosine monophosphate synthetase is essential for de novo purine synthesis. GMPS is the first partner gene of MLL on chromosome 3q and the first gene of this type in leukemia-associated translocations. (Blood. 2000;96:4360-4362)

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Gmps  (guanine monophosphate synthase)

Genes (Mus musculus)
Gmps  (guanine monophosphate synthetase)

Genes (Homo sapiens)
GMPS  (guanine monophosphate synthase)


Additional Information