RGD Reference Report - Mapping diabetes QTL in an intercross derived from a congenic strain of the Brown Norway and Goto-Kakizaki rats. - Rat Genome Database

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Mapping diabetes QTL in an intercross derived from a congenic strain of the Brown Norway and Goto-Kakizaki rats.

Authors: Collins, SC  Wallis, RH  Wilder, SP  Wallace, KJ  Argoud, K  Kaisaki, PJ  Bihoreau, MT  Gauguier, D 
Citation: Collins SC, etal., Mamm Genome. 2006 Jun;17(6):538-47. Epub 2006 Jun 12.
RGD ID: 1598974
Pubmed: (View Article at PubMed) PMID:16783636
DOI: Full-text: DOI:10.1007/s00335-005-0168-y

Genetic studies in experimental crosses derived from the inbred Goto-Kakizaki (GK) rat model of spontaneous diabetes mellitus have identified quantitative trait loci (QTL) for diabetes phenotypes in a large region of rat Chromosome (RNO) 1. To test the impact of GK variants on QTL statistical and biological features, we combined genetic and physiologic studies in a cohort of F(2) hybrids derived from a QTL substitution congenic strain (QTLSCS) carrying a 110-cM GK haplotype of RNO1 introgressed onto the genetic background of the Brown Norway (BN) strain. Glucose intolerance and altered insulin secretion in QTLSCS rats when compared with BN controls were consistent with original QTL features in a GK x BN F(2) cross. Segregating GK alleles in the QTLSCS F(2) cross account for most of these phenotypic differences between QTLSCS and BN rats. However, significant QTL for diabetes traits in both the QTLSCS and GK x BN F(2) cohorts account for a similar small proportion of their variance. Comparing results from these experimental systems provides indirect estimates of the contribution of genetic interactions and environmental factors to QTL architecture as well as locus and biological targets for future post-QTL mapping studies in congenic substrains.



Objects Annotated

QTLs
Niddm67  (Non-insulin dependent diabetes mellitus QTL 67)
Niddm68  (Non-insulin dependent diabetes mellitus QTL 68)
Niddm69  (Non-insulin dependent diabetes mellitus QTL 69)
Niddm70  (Non-insulin dependent diabetes mellitus QTL 70)

Objects referenced in this article
Strain BN.GK-(D1Wox18-D1Got254)/Ox null Rattus norvegicus
Strain GK/Ox null Rattus norvegicus

Additional Information