RGD Reference Report - The activation mechanism of alpha1 homomeric glycine receptors. - Rat Genome Database

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The activation mechanism of alpha1 homomeric glycine receptors.

Authors: Beato, M  Groot-Kormelink, PJ  Colquhoun, D  Sivilotti, LG 
Citation: Beato M, etal., J Neurosci. 2004 Jan 28;24(4):895-906.
RGD ID: 1598573
Pubmed: PMID:14749434   (View Abstract at PubMed)
PMCID: PMC6729805   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.4420-03.2004   (Journal Full-text)

The glycine receptor mediates fast synaptic inhibition in the spinal cord and brainstem. Its activation mechanism is not known, despite the physiological importance of this receptor and the fact that it can serve as a prototype for other homopentameric channels. We analyzed single-channel recordings from rat recombinant alpha1 glycine receptors by fitting different mechanisms simultaneously to sets of sequences of openings at four glycine concentrations (10-1000 microm). The adequacy of the mechanism and the rate constants thus fitted was judged by examining how well these described the observed dwell-time distributions, open-shut correlation, and single-channel P(open) dose-response curve. We found that gating efficacy increased as more glycine molecules bind to the channel, but maximum efficacy was reached when only three (of five) potential binding sites are occupied. Successive binding steps are not identical, implying that binding sites can interact while the channel is shut. These interactions can be interpreted in the light of the topology of the binding sites within a homopentamer.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
identical protein binding  IPIGlra1 (Rattus norvegicus)1598573homooligomerizationRGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Objects Annotated

Genes (Rattus norvegicus)
Glra1  (glycine receptor, alpha 1)
Glrb  (glycine receptor, beta)

Genes (Mus musculus)
Glra1  (glycine receptor, alpha 1 subunit)
Glrb  (glycine receptor, beta subunit)

Genes (Homo sapiens)
GLRA1  (glycine receptor alpha 1)
GLRB  (glycine receptor beta)


Additional Information