RGD Reference Report - Haemochromatosis protein is expressed on the terminal web of enterocytes in proximal small intestine of the rat. - Rat Genome Database

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Haemochromatosis protein is expressed on the terminal web of enterocytes in proximal small intestine of the rat.

Authors: West, AR  Thomas, C  Sadlier, J  Oates, PS 
Citation: West AR, etal., Histochem Cell Biol. 2006 Mar;125(3):283-92. Epub 2005 Oct 6.
RGD ID: 1582698
Pubmed: PMID:16208485   (View Abstract at PubMed)
DOI: DOI:10.1007/s00418-005-0060-6   (Journal Full-text)

The haemochromatosis protein (HFE) is an important regulator of body iron stores. In the liver, HFE is required for appropriate expression of hepcidin, a humoral mediator of iron absorption. HFE is also present in enterocytes, though its function in the intestine is unknown; it is not intrinsically required for iron absorption, but can augment iron absorption when over-expressed-independent of hepcidin regulation by the liver. In this study, an antibody was raised against rat HFE and validated by enzyme-linked immunosorbent assay, Western blot and quenching of antibody function by the immunising peptide. The sub-cellular location of HFE in enterocytes of iron-deficient and control rats was determined by double-labelling experiments with markers for the microvillus membrane, terminal web, early endosomes, lysosomes and the transferrin receptor. Parallel studies were performed for the primary iron absorption protein, divalent metal transporter 1 (DMT1). HFE co-localised exclusively with the terminal web of intestinal enterocytes. HFE expression was increased in iron deficiency, consistent with a second regulatory role for HFE in iron absorption, independent of hepcidin from the liver. DMT1 was localised primarily on the microvillus membrane, but did partially co-localise with HFE raising the possibility that the two proteins may interact to regulate iron absorption.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HfeRatcellular response to iron ion starvation  IEP  RGD 
HfeRatresponse to iron ion starvation  IEP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HfeRatapical part of cell  IDA terminal web of enterocytesRGD 
HfeRatmicrovillus membrane NOTIDA  RGD 
HfeRatterminal web  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hfe  (homeostatic iron regulator)


Additional Information