RGD Reference Report - Cross-linking of mitochondrial matrix proteins in situ. - Rat Genome Database

Send us a Message

Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Cross-linking of mitochondrial matrix proteins in situ.

Authors: D'Souza, SF  Srere, PA 
Citation: D'Souza SF and Srere PA, Biochim Biophys Acta. 1983 Jul 29;724(1):40-51.
RGD ID: 1582474
Pubmed: PMID:6409145   (View Abstract at PubMed)

Different cross-linkers (10 mM) of varying specificity and arm length were found to cross-link mitochondria matrix proteins in situ in 2 min at pH 7.4. As seen by SDS-polyacrylamide electrophoresis, the disappearance of individual protein bands was accompanied by concomitant appearance of polymeric aggregates that failed to enter the 4% spacer gel. The disorganization of the mitochondrial matrix infrastructure either by swelling or sonication of the mitochondria resulted in a decrease in the rate of cross-linking. Leakage of citrate synthase, malate dehydrogenase and fumarase was found to be reduced when cross-linked mitochondria were made permeable with toluene. On lysing the cross-linked mitochondria, a major part of the matrix protein (75%) was found to sediment with the membrane fraction. The activities of citrate synthase malate dehydrogenase and fumarase in rat liver mitochondria were also found to increase in the percipitates with concomitant decrease in their activities in the soluble matrix fraction. These results indicate that the cross-linkers enters the mitochondria and cross-links matrix proteins including Krebs cycle enzymes either to the mitochondrial membranes, or to themselves resulting in very large molecular weight complexes. These results are interpreted to mean that in liver mitochondria, the Krebs cycle enzyme are preferentially located near the membrane.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mdh2Ratmitochondrial matrix  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mdh2  (malate dehydrogenase 2)

Additional Information