RGD Reference Report - Nardilysin facilitates complex formation between mitochondrial malate dehydrogenase and citrate synthase. - Rat Genome Database

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Nardilysin facilitates complex formation between mitochondrial malate dehydrogenase and citrate synthase.

Authors: Chow, KM  Ma, Z  Cai, J  Pierce, WM  Hersh, LB 
Citation: Chow KM, etal., Biochim Biophys Acta. 2005 May 25;1723(1-3):292-301. Epub 2005 Mar 10.
RGD ID: 1582471
Pubmed: PMID:15809022   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbagen.2005.02.010   (Journal Full-text)

Gel filtration chromatography showed that nardilysin activity in a rat testis or rat brain extract exhibited an apparent molecular weight of approximately 300 kDa compared to approximately 187 kDa for the purified enzyme. The addition of purified nardilysin to a rat brain extract, but not to an E. coli extract, produced the higher molecular species. The addition of a GST fusion protein containing the acidic domain of nardilysin eliminated the higher molecular weight nardilysin forms, suggesting that oligomerization involves the acidic domain of nardilysin. Using an immobilized nardilysin column, mitochondrial malate dehydrogenase (mMDH) and citrate synthase (CS) were isolated from a fractionated rat brain extract. Porcine mMDH, but not porcine cytosolic MDH, was shown to form a heterodimer with nardilysin. Mitochondrial MDH increased nardilysin activity about 50%, while nardilysin stabilized mMDH towards heat inactivation. CS was co-immunoprecipitated with mMDH only in the presence of nardilysin showing that nardilysin facilitates complex formation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LipfRatmalate metabolic process acts_upstream_of_or_withinIDA PMID:15809022MGI 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CsRatmitochondrion located_inIDA PMID:15809022MGI 
LipfRatmitochondrion located_inIDA PMID:15809022MGI 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CsRatcitrate (Si)-synthase activity enablesIDA PMID:15809022MGI 
LipfRatmalate dehydrogenase activity enablesIDA PMID:15809022MGI 
NrdcRatpeptidase activity enablesIDA PMID:15809022MGI 
Mdh2Ratprotein binding  IPINrdc (Mus musculus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cs  (citrate synthase)
Lipf  (lipase F, gastric type)
Mdh2  (malate dehydrogenase 2)
Nrdc  (nardilysin convertase)

Additional Information