RGD Reference Report - Adriamycin induced myocardial failure in rats: Protective role of Centella asiatica. - Rat Genome Database

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Adriamycin induced myocardial failure in rats: Protective role of Centella asiatica.

Authors: Gnanapragasam, A  Yogeeta, S  Subhashini, R  Ebenezar, KK  Sathish, V  Devaki, T 
Citation: Gnanapragasam A, etal., Mol Cell Biochem. 2006 Jun 20;.
RGD ID: 1582470
Pubmed: PMID:16786185   (View Abstract at PubMed)
DOI: DOI:10.1007/s11010-006-9245-0   (Journal Full-text)

Generation of reactive oxygen species and mitochondrial dysfunction has been implicated in adriamycin induced cardiotoxicity. Mitochondrial dysfunction is characterized by the accumulation of oxidized lipids, proteins and DNA, leading to disorganization of mitochondrial structure and systolic failure. The present study was aimed to evaluate the efficacy of Centella asiatica on the mitochondrial enzymes; mitochondrial antioxidant status in adriamycin induced myocardial injury. Adriamycin (2.5 mg/kg body wt., i.p.) induced mitochondrial damage in rats was assessed in terms of decreased activities (p< 0.05) of cardiac marker enzymes (lactate dehydrogenase, creatine phosphokinase, amino transferases), TCA cycle enzymes (isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, malate dehydrogenase, respiratory marker enzymes (NADH-dehydrogenase, cytochrome-C-oxidase), mitochondrial antioxidant enzymes (GPx, GSH, SOD,CAT) and increased (p< 0.05) level of lipid peroxidation. Mitochondrial damage was confirmed by transmission electron microscopic examination. Pre-co-treatment with aqueous extract of Centella asiatica (200 mg/kg body wt, oral) effectively counteracted the alterations in mitochondrial enzymes and mitochondrial defense system. In addition, transmission electron microscopy study confirms the restoration of cellular normalcy and accredits the cytoprotective role of Centella asiatica against adriamycin induced myocardial injury. Our results demonstrated elevated oxidative stress and mitochondrial dysfunction in adriamycin treated rats. Moreover, on the basis of our findings it may be concluded that the aqueous extract of C. asiatica not only possesses antioxidant properties but it may also reduce the extent of mitochondrial damage.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MDH2Humancongestive heart failure  ISOMdh2 (Rattus norvegicus) RGD 
Mdh2Mousecongestive heart failure  ISOMdh2 (Rattus norvegicus) RGD 
Mdh2Ratcongestive heart failure  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mdh2  (malate dehydrogenase 2)

Genes (Mus musculus)
Mdh2  (malate dehydrogenase 2, NAD (mitochondrial))

Genes (Homo sapiens)
MDH2  (malate dehydrogenase 2)


Additional Information