RGD Reference Report - [Effects of myocardial ischemia reperfusion injury on L-arginine/nitric oxide system in rat heart] - Rat Genome Database

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[Effects of myocardial ischemia reperfusion injury on L-arginine/nitric oxide system in rat heart]

Authors: Zheng, HZ  Tang, CS  Su, JL  Wu, T 
Citation: Zheng HZ, etal., Sheng Li Xue Bao. 1999 Feb;51(1):25-30.
RGD ID: 1582431
Pubmed: PMID:11972171   (View Abstract at PubMed)

Ischemia reperfusion injury (IRI) model of rat heart was prepared by preperfusion for 15 min, then a suspension for 45 min and recycling reperfusion for 15 min with 30 ml KH buffer. The leakage of lactate dehydrogenase(LDH),protein, myoglobin and nitrite (NO2-) in the circular perfusion fluid were measured. Myocardial nitric oxide synthase(NOS) activity and L-arginine transport were observed. In the IR group, the leakage of LDH,protein, myoglobin and NO2- were increased respectively by 4.1,5.4,1 and 1.2 times(P<0.01) and NOS(tNOS, iNOS, cNOS) activity by 48.2%,43.2% and 52.1%,(P<0.01,respectively) as compared with the control group. L-arginine transport might be mediated by either high- or low-affinity transport system in cardiac tissue. In the IR group, L-arginine transport increased significantly with the V(max) being increased by 48% and 2 times respectively for the low-affinity and the high-affinity transport as compared with control. Michaelis constant (km) was decreased by 47.4% for low-affinity transport (P<0.05),but not significantly changed for the high-affinity transport. These results suggest that the increase of nitric oxide generation might result from the increased myocardial NOS activity and L-arginine transport during IRI.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Myocardial Reperfusion Injury  IDA 1582431 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mb  (myoglobin)


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