RGD Reference Report - Myoglobin gene expression attenuates hepatic ischemia reperfusion injury. - Rat Genome Database

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Myoglobin gene expression attenuates hepatic ischemia reperfusion injury.

Authors: Nitta, T  Xundi, X  Hatano, E  Yamamoto, N  Uehara, T  Yoshida, M  Harada, N  Honda, K  Tanaka, A  Sosnowski, D  Chance, B  Yamaoka, Y 
Citation: Nitta T, etal., J Surg Res. 2003 Apr;110(2):322-31.
RGD ID: 1582427
Pubmed: PMID:12788661   (View Abstract at PubMed)

BACKGROUND: Cellular functions are maintained by a continuous supply of ATP, which is supplied efficiently by mitochondrial oxidative phosphorylation. Since myoglobin, found in cardiac myocytes and red skeletal muscle, but not in the liver, facilitates oxygen diffusion under low oxygen conditions and enhances oxidative phosphorylation, this study seeks to enhance hepatic ATP levels and attenuate ischemia-reperfusion injury in rodent livers by adenovirus-mediated myoglobin expression. MATERIAL AND METHODS: After infecting Hep3B and rodent livers with adenovirus carrying CMV promoter sequences linked to the human myoglobin gene (AdCMVMyo), reverse transcriptase-PCR and immunodetection for myoglobin, and cellular and hepatic ATP levels were examined. The effect of myoglobin was evaluated in a hepatic ischemia-reperfusion model in the rat. RESULTS: Myoglobin expression was confirmed in Hep3B and rat livers after AdCMVMyo infection. The ATP levels in Hep3B cells and C57BL/6 mice livers 72 h after AdCMVMyo transfection were significantly higher than control levels and those after adenovirus-mediated beta-galactosidase transfection. Finally, expression of myoglobin attenuated ischemia-reperfusion injury in the rat liver. CONCLUSION: These results indicate that myoglobin gene transfer to the liver enhanced ATP levels both in vitro and in vivo and might be a novel strategy to reduce ischemia-reperfusion injury.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MBHumanReperfusion Injury  IDA  RGD 
MbRatReperfusion Injury  ISOMB (Homo sapiens) RGD 
MbMouseReperfusion Injury  ISOMB (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mb  (myoglobin)

Genes (Mus musculus)
Mb  (myoglobin)

Genes (Homo sapiens)
MB  (myoglobin)


Additional Information